纳米载银无机抗菌剂对义齿基托树脂细胞毒性的影响

目的研究纳米载银无机抗菌剂对义齿基托树脂细胞毒性的影响。方法纳米载银无机抗菌剂FUMAT T200-4,按0%(对照组)、1%、2%、3%、4%、5%、7%、10%(V/V)比例添加在加热固化型义齿基托树脂中并分组,采用MTT法检测各组基托树脂对传代培养的小鼠成纤维细胞L-929的细胞毒性,比较抗菌剂添加比例对基托树脂细胞毒性的影响。结果作用于小鼠成纤维细胞后2、4、7 d,各抗菌剂添加比例组基托树脂毒性反应为0级或1级,小鼠成纤维细胞相对增殖率分别为93.4%1~05.9%、114.2%~147.1%和131.8%1~48.8%。结论添加低浓度纳米载银无机抗菌剂FUMAT T200-4(添加比例≤10%)的基托树脂,无明显细胞毒性,具有较好的生物安全性。

Influence of Nano-Silver Base Inorganic Antimicrobial on Cytotoxicity of Denture Base Resin

Objective To explore the influence of nano-silver base inorganic antimicrobial on cytotoxicity of nano-antimicrobial denture base resin. Methods The experimental groups of denture base resin were mixed with the nano-silver base inorganic antimicrobial FUMAT T200-4,whose incorporating doses were 0%(control),1%,2%,3%,4%,5%,7% and 10%(V/V).Cytotoxicity in vitro on rat's fibroblast was assayed with MTT method,and the grades were compared. Results After 2,4 and 7days,the grades of cytotoxicity were 0 or 1,and the cell rela-(tive growth) rates of experimental groups were 93.4%-105.9%,114.2%-147.1% and 131.8%-148.8%.(Conclusion)When the incorporation ratios of the nano-silver base inorganic antimicrobial FUMAT T200-4 were 10% or fewer,the nano-antimicrobial denture base resin does not have cytotoxicity on small rat's fibroblast and shows good biocompatibility.