PLA-PEG复合支架材料体外细胞相容性的实验研究

目的:探索新型PLA-PEG(polylactic acid-polyethylene glycol block copolymers)复合支架材料体外细胞相容性。方法:支架材料分为实验组A(PEG/PLA为16∶0)、实验组B(PEG/PLA为13∶0)、对照组C,单纯聚乳酸(polylacticacid,PLA)。贴壁法培养兔骨髓基质细胞(bone marrow stromal cells,BMSCs),诱导生成成骨细胞,接种于支架材料上。通过观察种子细胞在支架材料上的吸附迁移、生长增殖情况,验证各组细胞材料复合体的成骨活性,比较分析不同支架材料之间的差异。结果:兔成骨细胞在新型PLA-PEG三维支架材料表面分布较为均匀,生物学行为活跃。实验组A与B的细胞粘附及增殖能力均强于对照组(P<0.05)。结论:三种支架均具有较好的细胞相容性,但实验组A与B的细胞相容性更好,更适宜作为支架材料与成骨细胞共同培养,以获得具有成骨能力的三维立体结构组织工程骨。

In vitro Experiment on Cell Compatibility of a New PLA-PEG Scaffold

Objective: To study the cell compatibility and constructive method of tissue engineering bone from a newly PLA-PEG scaffold in vitro.Methods: Bone marrow stromal cells(BMSCs) obtained from rabbits were induced into osteoblasts and co-cultured with new PLA-PEG scaffold A: PEG /PLA(1∶60),B: PEG /PLA(1∶30)] and PLA alone as control.Morphological characteristics,cell proliferation rate,and alkaline phosphatase activities from attached MSC were detected.The differences between groups was compared and analyzed.Results: Induced osteoblasts could attache to 3 scaffolds,and normally grew,proliferated,and functioned actively on them.Moreover,experimental groups showed superior in adhensive and proliferative abilities than control group(P<0.05).Conclusion: The 3 scaffolds have good cell compatibility.Moreover,PEG /PLA is superior than PLA and appeared to be more adaptable for co-cultured with MSC in vitro to harvest tissue-engineering bone.