天津地区IL-1 Ra基因内含子2可变串联重复序列与脓毒症的相关性研究

目的：探讨白细胞介素-1受体拮抗剂（IL-1 Ra）基因第2内含子中可变串联重复序列（VNTR）多态性与脓毒症的相关关系。方法：应用PCR法，观察90例脓毒症患者、120例健康对照者的IL-1 Ra基因VNTR多态性的分布。结果：两组人群中共发现了IL-1 RNI／I、IL-1RN Ⅰ／Ⅱ、IL- 1RNI／Ⅳ3种基因型，以I型等位基因为主，未发现Ⅲ型和Ⅴ型。正常对照组中Ⅱ型等位基因和Ⅰ／Ⅱ基因型的分布频率与脓毒症组相比明显增加（8％vs2％，P〈0．05；17％vs4％，P〈0．05）。另外，不携带Ⅱ型等位基因的基因型（Ⅰ／Ⅰ和Ⅰ／Ⅳ）和非Ⅱ型等位基因携带者（Ⅰ，Ⅳ）与携带Ⅱ型等位基因的基因型（Ⅰ／Ⅱ）和Ⅱ型等位基因携带者相比发生脓毒症的相对危险度分别为4．3和4．0。结论：I-1RN Ⅱ型等位基因可能在脓毒症的发生发展过程中具有潜在的保护作用。

Association of sepsis and the variable number of tandem repeat polymorphism in intron two of interleukin-1 receptor antagonist gene in Tianjin population

Objective： To investigate the association of sepsis and variable number of tandem repeats （VNTR） polymorphism in the second intron of IL-1Ra gene. Methods： The distribution of VNTR polymorphism in IL-1Ra gene was observed by the method of PCR in 90 patients of sepsis and 120 healthy controls. Results： Three kinds genotypes, IL-1RN I / I ,IL-1RN I/II and IL-1RN I /IV were found in case group and healthy control group, and type- I allele were the mainly.The frequency of the type H alleles and the I / II genoty- pe in the normal control group was significantly increased compared with that in the sepsis group （0.08vs0.02, P〈0.05; 0.17 vs 0.04, P〈0.05）, also, the relative risk of occurring sepsis between genotype （ I / I, I/IV） and （ I/II ） was 4.3 and the relative risk of acurring sepsis between （ I / IV） and （ II ） was 4.0. Conclusion： IL-1RN II alleles may have the potentially protective effect in the development of sepsis.