铁皮石斛原球茎与原药材免疫调节作用的比较研究

目的:比较研究铁皮石斛组织培养原球茎与原药材对免疫功能的作用及急性毒性反应。方法;用白细胞计数法、免疫器官重量法、碳粒廓清法、淋巴细胞转化试验来研究药物对环磷酰胺造成小鼠免疫功能低下的对抗作用;以最大耐受量的方法研究药物的急性毒性。结果:铁皮石斛组织培养原球茎有升高环磷酰胺模型小鼠外周血白细胞数,增加模型鼠胸腺或脾脏指数,增强巨噬细胞的吞噬功能,促进模型鼠体内淋巴细胞转化的作用。口服给予药物量54.56 g生药/kg,小鼠仍能耐受。这些作用与原药材相似。结论:铁皮石斛组织培养原球茎与原药材均具有提高免疫功能作用,两者作用强度相似,最大耐受量相当于人临床用药量的227倍。

Comparative Study of Tissue Cultured Dendrobium Protocorm with Natural Dendrobium candidum on Immunological Function

Objective: To compare the immunological function and acute toxicity of the tissue cultured protocorm from Dendrobium candidum with natural medicinal materials from Dendrobium candidum. Methods: The effect on immunological function was examined by counting white blood cells, weighing the weight of immune organs,and using carbon granules clearance and lymphocyte transformation test in mice treated with cyclophospamide.The acute toxicity was studied by giving maximum tolerated dose.Results:The tissue cultured protocorm could increase the quantity of white blood cells, the ratios of thymus weight to body weight and spleen weight to body weight, promote the function of phagocytes and enhance the lymphocyte transformation rate.The mice could tolerate the dose of 54.56 g/kg(dried herbs)by oral administration.The functions were similar to those of natural medicinal materials from Dendrobium candidum.Conclusion: Both tissue cultured protocorm and natural medicinal materials from Dendrobium candidum could improve immunological function with similar potency.The maximum tolerated dose was 227 times as high as the effective clinical.