| VEGF165 对脱细胞真皮基质血管化影响的研究 |
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| 引用本文： | 李菲菲,米立国.VEGF165 对脱细胞真皮基质血管化影响的研究[J].大连医科大学学报,2009,31(1):18-22. |
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| 作者姓名： | 李菲菲 米立国 |
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| 作者单位： | 1. 大连医科大学,人体解剖学教研室,辽宁,大连,116044
2. 河北医科大学,人体解剖学教研室,河北,石家庄,050000 |
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| 基金项目： | 河北省卫生厅科研基金 |
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| 摘 要： | 目的]观察十二烷基硫酸钠(sodium dodecyl sulfate,SDS)和NaOH复合消蚀法制备的胎儿脱细胞真皮基质(ADM)的组织相容性,以及血管内皮生长因子(VEGF)对ADM血管化的影响。方法]将足月胎儿皮肤用SDS和NaOH复合消蚀,制备ADM(厚0.3 mm)。用重约300 g的SD雄性大鼠45只,随机分为ADM、ADM+rAAV/LacZ和ADM+rAAV/VEGF1653组进行真皮片皮下包埋实验。术后的2、4、6周分别取材,经HE染色或以VEGF165、基质金属蛋白酶-1(MMP-1)、组织型基质金属蛋白酶抑制剂-1(TIMP-1)的单克隆抗体进行免疫组织化学染色,检测3种蛋白质在移植后的皮肤中的表达情况。观察并比较各组中ADM血管密度,进行图像处理和统计学分析。结果]ADM无细胞成分,胶原纤维结构规整;术后2周包埋ADM组织中可见到新生血管,及VEGF阳性细胞;无明显的炎性细胞浸润。ADM临近组织中MMP-1和TIMP-1均明显表达,MMP-1的表达高于TIMP-1;术后4周各组细胞数量和新生血管均高于2周。此时MMP-1的表达高于TIMP-1,但TIMP-1的表达较2周时有所增...
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| 关 键 词： | 脱细胞真皮基质 血管内皮生长因子 血管化 |
Effects of VEGF165 on vascularization of acellular dermal matrix |
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| LI Fei-fei and MI Li-guo.Effects of VEGF165 on vascularization of acellular dermal matrix[J].Journal of Dalian Medical University,2009,31(1):18-22. |
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| Authors: | LI Fei-fei and MI Li-guo |
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| Institution: | LI Fei-fei 1,MI Li-guo2(1.Department of Anatomy,Dalian Medical University,Dalian 116044,China,2.Department of Anatomy,Hebei Medical University,Shijiazhuang 050000,China) |
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| Abstract: | Objective] To observe the biocompatibility of acellular dermal matrix(ADM) prepared by SDS and NaOH maceration,and VEGF165 on the effects of the vascularization of ADM.Methods] Randomly 45 mice were devided into three groups-ADM vacnity group,ADM+rAAV/LacZ control group and ADM+rAAV/VEGF165 experiment group.ADMs were embed between skins and skin-muscles on the backs of mice.2,4,6 weeks after operations mice skins were drawn the materials from back section.Then they were characterized with HE staining and ... |
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| Keywords: | acellular dermal matrix vascular endothelial growth factor vascularization |
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