| 绒毛间期核荧光原位杂交中探针类型选择 |
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| 引用本文： | 乔惠珍 苏光. 绒毛间期核荧光原位杂交中探针类型选择[J]. 内蒙古医学院学报, 1996, 18(2): 74-77 |
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| 作者姓名： | 乔惠珍 苏光 |
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| 作者单位： | Department of Gynecology and Obstetrics，First Affilated Hospital |
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| 摘 要： | 本文选择了α重复序列、染色体特异性ＤＮＡ及Ｃｏｓｍｉｄ为探针，分别与绒毛间质细胞间期核杂交，结果表明：绝大多数间期核中均见杂交信号，２１／１３α重复序列探针用于未培养的绒毛间质细胞杂交信号最为特异，能有效的检出染色体非整倍体，从而建立了一种快速而准确的产前筛选胎儿非整倍体的方法，成为细胞学一项极佳的辅助手段。
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| 关 键 词： | 荧光原位杂交，同期FISH，产前诊断 |
AN ASSAY ON SELECTION OF PROBS FOR INTER FISH |
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| Qiao Huizhen, Su Guang. AN ASSAY ON SELECTION OF PROBS FOR INTER FISH[J]. Acta Academiae Medicinae Neimongol, 1996, 18(2): 74-77 |
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| Authors: | Qiao Huizhen Su Guang |
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| Abstract: | We have selected alpha satellite repetitive probe specific for chromo.some 21 and13, chromosome-specific painting probe and cosmid probe, hybridizing with the uncultured in-terphase cells from mesenchymal chorionic villus, in order to assay the feasibility of the materialfor inter - FISH. The results indicate that this cells can be used for inter-FISH and produce hy-bridization signals. However, 21/13 alpha-satellite repetitive probe gave the most specific signals for mesenchymal chorionic villus. It can detect chromosome aneuploidy effectively and can beapplied to prenatal diagnosis. |
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| Keywords: | FISH inter-FISH prenatal diagnosis. |
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