热休克反应对大鼠感染性脑水肿细胞因子和一氧化氮的影响及意义

为研究IL 1β、TNF α及NO在感染性脑水肿时的变化和热休克反应对它们的影响 ,探讨热休克反应对大鼠感染性脑水肿的保护作用是否与其有关。本实验采用ELISA法和Griess法分别检测各组大鼠脑组织匀浆中IL 1β、TNF α及NO的含量。大鼠分为 3组 :①正常对照组 (NS ) ;②感染性脑水肿组 (PB ) ;③热休克处理组 (HS +PB )。每组又分为 4h、 8h、 2 4h三个时间点。结果显示 :与NS组比较 ,4h、 8h、 2 4hPB组的TNF α和NO含量明显增加 ,以 8h为最明显 (P <0 0 1或P <0 0 5 ) ;而在 4h、 8hPB组中 ,脑组织IL 1β含量明显增高 ,以 8h增高最明显 (P <0 0 1或P <0 0 5 ) ,2 4hPB组IL 1β含量明显下降。热休克反应能降低IL 1β、TNF α和NO在脑组织中的含量 ,在统计学上均有显著性意义 (P <0 0 1或P <0 0 5 )。以上结果表明IL 1β、TNF α及NO参与了感染性脑水肿的病理过程 ,热休克反应能减轻感染性脑水肿可能与其抑制IL 1β ,TNF α及NO生成有关

Effect of Heat Shock Response on Cytokines and NO Levels in the Brain Tissue of Infectious Brain Edema in Rats

To study the changes of IL1β,TNFα and NO levels in the brain of rats with infectious edema and their relation to the heat shock reaction (HSR),the concentrations of IL1β,TNFα and NO in the brain homogenates of rats were determined by ELISA and Griess reaction In this experiment,rats were randomly divided into 3 groups:(1) Normal control (NS) group;(2)Infectious brain edema (PB) group;(3)Heat shock treatment group (NS+PB);and animals in each group of experiment were killed at 4h,8h and 24h after injections of PB or NS The results showed that the concentration of TNFα and NO were significantly elevated in PB group than in NS group at various time points,and the concentrations of IL1β were significantly increased in PB group in comparison with those of NS group at 4h and 8h The HSR could reduce the contents of IL1β,TNFα and NO in the brain tissues These data implicate that IL1β,TNFα and NO are all involved in the pathogenesis of infectious brain edema The HSR can lessen the occurrence of edema,and this might be related with the inhibition of the productions of these substances in the brain tissue