| Gene Expression of Adiponectin and Adiponectin Receptor 1 in Type 2 Diabetic Rats and the Relationship with the Parameters of Glucose and Lipid Metabolism |
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| 作者姓名： | 姚辉 林汉华 王宏伟 张龙江 黄晓燕 夏治 |
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| 作者单位： | Department of Pediatrics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Pediatrics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Pediatrics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Pediatrics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Pediatrics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Pediatrics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China，Wuhan 430030，China |
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| 摘 要： | Adiponectin is an adipocyte-derived factor ,which plays pivotal roles of the insulin-sensitizing,anti-inflammatory ,and atheroprotective . Two adi-ponectin receptor types were recently identified:AdipoR1 is abundantly expressed in muscle ,whereas AdipoR2 is predominantly expressed inthe liver1].They are thought totransmit effects ofadiponectin . We examined serumadiponectin, mR-NAlevels of adiponectinin adipose tissue and mR-NAlevels of AdipoR1 inthe muscles of type 2 dia-betic rats ,to …
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| 关 键 词： | 基因表达 脂肪代谢 骨骼肌 2型糖尿病 葡萄糖 |
| 收稿时间： | 23 March 2005 |
Gene expression of adiponectin and adiponectin receptor 1 in type 2 diabetic rats and the relationship with the parameters of glucose and lipid metabolism |
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| Yao Hui,Ling Hanhua,Wang Hongwei,Zhang Longjiang,Huang Xiaoyan,Xia Zhi.Gene Expression of Adiponectin and Adiponectin Receptor 1 in Type 2 Diabetic Rats and the Relationship with the Parameters of Glucose and Lipid Metabolism[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(3):285-288. |
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| Authors: | Yao Hui Ling Hanhua Wang Hongwei Zhang Longjiang Huang Xiaoyan Xia Zhi |
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| Institution: | Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | In order to confirm whether the mRNA levels of adiponectin in adipose tissue and mRNA levels of AdipoR1 in the skeletal muscles were correlated with the serum parameters of glucose and lipid metabolism and to clarify the regulation of adiponectin receptor gene expression in diabetic states, serum adiponectin, mRNA levels of adiponectin in adipose tissue and mRNA levels of AdipoR1 in the skeletal muscles were examined in type 2 diabetic rats. The model of type 2 diabetes was prepared by feeding high fat diet and injecting low dosage of streptozotocin (STZ). The diabetic rats were screened out by oral glucose tolerance test. One group of type 2 diabetic rats received rosiglitazone. The serum adiponectin concentration was detected by using ELISA and mRNA levels were examined by RT-PCR. The serum adiponectin levels and mRNA levels of adiponectin in adipose tissue of type 2 diabetic rats were significantly decreased as compared with the normal control rats (P<0.05, P<0.01 respectively). No siglificant changes were observed in the expression of adiponectin receptor 1 in the skeletal muscle of type 2 diabetic rats. The mRNA levels of adiponectin in adipose tissue were reversely correlated with serum insulin (r=-0.66, P<0.05), triglyceride (r=-0.58, P<0.05), cholesterol (r=-0.49, P<0.05), interleukin-6 (r=-0.49, P<0.05) and tumor necrosis factor (r=-0.43, P<0.05). The expression of adiponectin receptors was not altered in the skeletal muscle of Type 2 diabetic rats. The decreased serum adiponectin was caused by the decreased expression of adiponectin mRNA in adipose tissue rather than the adiponectin receptors in the skeletal muscle, which could be improved by rosiglitazone. |
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| Keywords: | adiponectin adiponectin receptor diabetes mellitus non-insulin-dependent rosiglitazone |
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