肝癌患者血清中黄曲霉毒素白蛋白加合物与肝肾功能的关系

目的]评价肝癌患者AFB 1-alb加合物的暴露水平以及与肝肾功能的关系.方法]采用ELISA试剂盒分析82例肝癌患者、31例肝炎患者和51名健康人血清中的AFB1-alb加合物,同时使用全自动生化仪检测不同AFBl-alb加合物水平肝癌患者的肝肾功能,核磁共振成像(MRI)验证肝损伤.结果] AFB1-alb加合物在肝癌患者血清中的水平高于健康人,两者之间差别有统计学意义(P＜0.05).肝癌和肝炎两组患者之间AFB1-alb加合物水平无明显差异AFB1-alb加合物的暴露水平与身体质量指数有关,而与年龄、性别及居住地无关.血清中的AFB1-alb加合物与总胆红素、谷酰转肽酶、肌酐、尿酸、甘油三脂、胆固醇相关,而与白蛋白、转氨酶、碱磷酶、尿素无明显相关.AFB1-alb加合物高水平组MRI显示脂肪变性的比例高于低水平组(48.8％vs 25.6％),两组之间差异有统计学意义.结论]肝癌患者血清中的AFB 1-alb加合物高暴露水平影响肝肾功能,AFB1-alb加合物可能成为肝癌诊断的血清学潜在标志物.

Detection of Serum AFB1-alb Adduct and Its Association with Hepatic and Renal Functions in Patients with Hepatocellular Carcinoma

Purpose] To investigate the level of AFB1-alb adduct in serum samples from hepatocellular carcinoma patients and its association with hepatic and renal functions.Methods] Blood samples were collected from 82 hepatocellular carcinoma patients,31 hepatitis patients and 51 healthy controls.The level of AFB1-alb adduct was detected by ELISA.AFB1-alb adduct,albumin,total bilirubin,AST,ALT,ALP,GGT,creatinine,TG,CHOL and Urea were detected by automatic biochemical analyzer,and hepatic lesion was evaluated by magnetic resonance imaging (MRI).Results] Hepatocellular carcinoma patients had higher AFB1-alb adduct levels than healthy control,with significant difference(P＜0.05).There was no remarkable difference between hepatocellular carcinoma and hepatitis patients.AFB1-alb adduct levels did not show significant correlations with age,gender and residence.Additionally,there was significant relationship between body mass index and AFB1-alb adduct.Patients with AFB1-alb adduct levels greater than average had significantly elevated level of total bilirubin,GGT,creatinine,Uric,TG and CHOL.Nevertheless,the level of albumin,ALT,AST,ALP and Urea showed no correlation with the level of AFB1-alb adduct.High AFB 1-alb adduct level group by MRI showed that the proportion of hepatic lesion was higher than that of the low group(48.8％ vs 25.6％),with significant difference(P＜O.05).Conclusions] High exposure levels of serum AFB1-alb adduct could affect the functions of liver and kidney in hepatocellular carcinoma patients.It implies that AFB1-alb adduct might be a potential biomarker in the diagnosis of hepatocellular carcinoma.