Bmi-1和P16在大肠肿瘤中的表达及临床意义

目的 研究大肠肿瘤组织中Bmi-1、P16蛋白表达情况及临床意义。方法采用免疫组织化学方法分别检测Bmi-1、p16蛋白在60例大肠癌、30例大肠腺瘤及20例正常大肠粘膜组织中的表达情况及其与大肠癌临床病理特征及患者生存率的关系。结果Bmi-1及p16蛋白在大肠癌中表达明显高于腺瘤组及正常组（P＜0．05）；采用卡方检验显示，Bmi-1蛋白高表达与有无远处转移密切相关（P〈0．01），与TNM分期有关（P＜0．05），而p16蛋白表达与各临床病理特征无关（P〉0．05）；Kaplan—Meier生存分析显示Bmi-1蛋白高表达患者生存率明显低于低表达患者（P＜0．05），而p16蛋白表达阳性患者生存率与p16蛋白表达阴性患者生存率差异无统计学意义（P〉0．05）。结论Bmi-1蛋白表达与大肠癌的发生、发展及预后关系密切，可作为评估大肠癌预后的参考指标；p16蛋白表达可能与大肠癌发生有一定关系，但与肿瘤转移及预后无关。

Expression and significance of Bmi-1 and P16 in colorectal carcinoma

Objective To investigate the expression and significance of Bmi-1 and p16 in colorectal carcinoma in human CRC. Methods Sixty CRC,thirty adenomas and twenty normal colorectal mucosal tissues were used in this study. The expression of Bmi-1 and pl6 was detected by immunohistochemical assay. Results The expression of Bmi-1 and p16 was significantly high in CRC,compared with adenomas and normal colorectal mucosal tissues （P〈0.05）. The overexpression of Bmi-1 protein in CRC was obviously associated with distant metastasis（P〈0.01 ）and TNM stage（P〈0.05）,but the positive expression of p16 protein in CRC was not significantly associated with clinicopathological features （P〉0.05）. Kaplan-Meier survival analysis showed that the overexpression of Bmi-1 reduced significantly survival of CRC patients. （P〈0.05）.However the expression of p16 protein in CRC was not significantly associated with the survival. Conclusion The overexpression of Bmi-1 protein is significantly correlated with tumorigenesis,metastasis and prognosis of CRC. Bmi-1 might be used for predicting the prognosis of CRC. The positive expression of p16 protein is possibly associated with tumorigenesis ,but not with metastasis and prognosis of CRC.