Abstract: | The research reported showed that: the stability of cell‐free HVT vaccine, lyophilized and sealed under vacuum, is very high: after one year at 4°‐6°C and after 10 days at 37°C the titre decreased only about 0.4 log10; the stability of the vaccine, when reconstituted for use in the diluent (sucrose‐phosphate buffer), is high (80%) for at least 2 hours; the degree of protection, found in 3 tests, was about 90% with an inoculum of 20 to 100 pfu, and the PD50 was 4.9 pfu of virus per chick. |