特异性CTL胞毒作用与HLA型别关系探讨

目的：探讨特异性CTL胞毒作用与靶细胞的HLA型别关系。方法：本实验以特定HLA型别，EB病毒转化的B淋巴母细胞（EBV－LCL）与同种异体单个核细胞（PBMC）混合培养，激活同种抗原特异性细胞性T细胞（CTL），观察其对携带不同HLA型别的EBV－LCL靶细胞的杀伤活性。结果：用EBV－LCL1致敏的效应细胞1对EBV－LCL2的杀伤效应比对EBV－LCL3强，用EBV－LCL2致敏的效应细胞2对EBV－LCL1的杀伤效应比对EBV－LCL3强，用EBV－LCL3致敏的效应细胞3对EBV－LCL1，2几乎无杀伤效应。结论：特异性CTL对不同HLA型别的靶细胞的胞毒作用存在差异，这不仅仅与HLA型别关联，而可能取决于抗原肽与MHC所组成的抗原综合信息。

Investigaton of the relationship between cytotoxicity of specific CTL and HLA allotype

ve To investigate the relationship between cytotoxicity of specific CTL and HLA allotype. Methods B lympho-blastoid cell lines transformed by the Epstein-Barr-Virus (EBV-LCL), having different HLA allotype were used as stimulators to stimulate the peripheral blood mononuclear cells (PBMC) to proliferate into cytotoxic T lymphocytes (CTL), and then the specific CTL was used to do cyto-toxicity experiments towards different targets (EBV-LCL as mentioned above). Results When the EBV-LCLl was used as stimulator, cytoto-xicity of the CTL obtained from the mixed lymphocyte culture towards EBV-LCL2 was stronger than that towards EBV-LCL3; when the EBV-LCL2 was used as stimulator, cytoto-xicity of the CTL towards EBV-LCLl was stronger than that towards EBV-LCL3. When EBV-LCL3 was used as stimulator, cytotoxicity of the CTL towards both EBV-LCL2 and EBV-LCLl were very low. Conclusion There are differences in cytotoxicity of the CTL to different targets with different HLA types , which are not just correlated with HLA type, but also correlated with comprehensive information of MHC-peptide complex.