肠道T细胞淋巴瘤中的EB病毒感染和T细胞内抗原1的表达

目的 探讨ＥＢ病毒感染在肠道Ｔ细胞淋巴瘤发病中的意义。方法 用ＥＢＥＲ１／２原位杂交及三步ＡＢＣ法免疫组织化学染色技术,观察２４例肠道Ｔ淋巴瘤患者中ＥＢ病毒感染及Ｑ细胞内抗原（ＴＩＡ－１）抗原表达情况,选用的抗体有ＴＬＡ－１,ＬＭＰ－１,ＣＤ３,ＣＤ２０,ＣＤ３０和ＣＤ４５ＲＯ等。

Epstein-Barr virus infection and expression of T-cell intracellular antigen-1 (TIA-1) in intestinal T-cell lymphoma

Objective To study the status of Epstein Barr virus (EBV) infection and the expression of TIA 1 antigen in 24 cases of intestinal T cell lymphomas. Methods In situ hybridization for EBER1/2; immunohistochemical staining by three step ABC technique for TIA 1, LMP 1, CD30, CD45RO, CD3 AND CD20. 19 cases of intestinal B cell lymphomas were used for comparison. Results (1) Of the 24 intestinal T cell lymphoma cases, 23 were pleomorphic, medium to large cell type, and one cases was small cell type; in 20 of the 24 cases EBER1/2 signals were detected in the tumor cells; in 4 of the 20 EBER1/2 positive cases, tumor cells expressed LMP 1; EBER1/2 was not detected in 19 cases of intestinal B cell lymphomas. (2) in 23 of the 24 cases, TIA 1 was expressed in most tumor cells, but not in the only case of small cell type; TIA 1 was negative in tumor cells of all 19 cases of intestinal B cell lymphomas. Conclusions A high level of EBV infection was present in this group of intestinal T cell lymphomas, the expression of TIA 1 may be related to its cell origin. Intestinal T cell lymphoma may be an EBV associated extranodal NK/T cell lymphoma.