Culture-independent species typing of neotropical Leishmania for clinical validation of a PCR-based assay targeting heat shock protein 70 genes |
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| Authors: | Garcia Lineth Kindt Ann Bermudez Hernan Llanos-Cuentas Alejandro De Doncker Simonne Arevalo Jorge Wilber Quispe Tintaya Kelly Dujardin Jean-Claude |
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| Institution: | Prince Leopold Institute of Tropical Medicine, Molecular Parasitology, Antwerp, Belgium. |
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| Abstract: | PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis. |
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