水通道蛋白过表达对脊髓损伤大鼠便秘的影响

目的 探讨脊髓损伤大鼠结肠水通道蛋白(AQPs)的表达及其与粪便含水率的关系。方法 将48只雌性Sprague-Dawley大鼠分为对照组(n = 24)和脊髓损伤组(n = 24)。脊髓损伤组行T₈横断脊髓损伤,对照组仅行椎板切除术。于造模前,造模后第1、3、7、14、28天,采用Basso-Beattie-Bresnahan (BBB)评分评价后肢运动功能;于术前,术后第3、14、28天检测粪便含水率;于术后第3、14、28天取结肠标本,免疫组化检测AQP1、AQP3和AQP4的表达。结果 脊髓损伤组术后BBB评分(t > 69.230, P< 0.001)和粪便含水率(t > 5.814, P< 0.001)均显著低于对照组。术后第3、14、28天,脊髓损伤组结肠AQP1、AQP3和AQP4表达均明显高于对照组(|t|> 5.165,P < 0.01)。脊髓损伤后AQPs表达与粪便含水率呈显著负相关( r = -0791~-0.730, P< 0.001)。结论 脊髓损伤后大鼠结肠AQPs表达上调,可能与脊髓损伤后结肠过度吸收水分有关。

Aquaporin Over-expression for Constipation in Rats with Spinal Cord Injury

Objective To investigate the relation between aquaporins(AQPs)and fecal water content in rats with spinal cord injury.Methods A total of 48 female Sprague-Dawley rats were divided into control group(n=24)and spinal cord injury group(SCI group,n=24).SCI group underwent transection at T8,while the control group was only subjected to laminectomy.Posterior limb function was assessed by Basso-Beattie-Bresnahan(BBB)score before modeling and on the 1 st,3 rd,7 th,14 th and 28 th day after SCI.Fecal water content was measured before modeling and on the 3 rd,14 th and 28 th day after SCI.Colon specimens were collected to detect the expression of AQP1,AQP3 and AQP4 by immunohistochemistry on the 3 rd,14 th and 28 th day after SCI.Results The BBB score was significantly lower in SCI group than in the control group(t>69.230,P<0.001)after SCI,as well as the fecal water content(t>5.814,P<0.001).The expression of AQP1,AQP3,and AQP4 in the colon was higher in SCI group than in the control group(|t|>5.165,P<0.01)on the 3 rd,14 th,and 28 th day after SCI.The expression of AQPs negatively correlated with fecal water content(r=-0791^-0.730,P<0.001).Conclusion The expression of AQPs in the colon of rats after SCI was up-regulated,which was correlated with excessive water absorption after SCI.