脱蛋白液对异种骨移植替代物生物学特性影响

目的研究不同脱蛋白时间对异种骨移植替代物细胞毒性、生物相容性和体内降解性的影响。方法采用脱蛋白液分别浸泡牛松质骨0、24、36、48和60h，通过与细胞共培养观察异种骨对骨髓基质细胞的细胞毒性，通过异种动物体内埋置观察其生物相容性和降解性。结果脱蛋白液处理48h和60h组细胞相对增殖率与空白对照组间无显著性差异（P〉0．05），细胞相容性最好，细胞毒性分级为0级；扫描电镜和组织学观察结果显示脱蛋白液处理48h组早期（4周内）即出现细胞长入软组织，具有良好的生物相容性；X线片、扫描电镜、能谱分析和显微CT观察显示48h组支架结构降解性明显优于其他组（P〈0．05），12周后48h组支架几乎完全降解。结论脱蛋白液处理48h后，异种骨移植替代物的细胞毒性最低，生物相容性和降解性达到最佳状态。

The influence of deproteinating fluid on the biological characteristics of xenograft bone substitute

Objective To investigate the influences of different timing of deproteinating on the cytotoxicity,biocompatibility and degradability of xenograft bone substitute.Methods Bovine cancellous bone granules were immersed into deproteinating fluid for 0h,24h,36h,48h and 60h respectively.The cytotoxicity of these granules was observed through the co-culture with hMSCs,while the biocompatibility and the degradability were observed through the imbedment into muscle pouches of mouse.Results The relative growth rates(...