HPLC-UV法测定草决明中五种蒽醌类化合物含量及其指纹图谱的建立 |
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引用本文: | 何永志,严治学,任晓亮,常艳旭.HPLC-UV法测定草决明中五种蒽醌类化合物含量及其指纹图谱的建立[J].河北医学,2014,0(12):1937-1941. |
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作者姓名: | 何永志 严治学 任晓亮 常艳旭 |
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作者单位: | 何永志 (天津中医药大学中药学院,天津,300193); 严治学 (天津中医药大学中药学院,天津,300193); 任晓亮 (天津中医药大学中药学院,天津,300193); 常艳旭 (天津中医药大学中药学院,天津,300193); |
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基金项目: | 基金项目:国家自然科学基金(青年基金).编号:81102732 |
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摘 要: | 目的:建立一种同时测定草决明中五种蒽醌类化合物含量的HPLC方法及草决明HPLC指纹图谱。方法:采用Hypersil C18色谱柱(4.6mm×250mm,5μm),以乙腈-0.05%甲酸水溶液为流动相,梯度洗脱;流速1.0mL/min,柱温:30℃,检测波长254nm。采用评价软件(2004A)进行数据分析,对来自加纳和中国13个地区,共22种生、炒草决明建立了HPLC 指纹图谱,并进行相似度计算。结果:方法的灵敏度、线性、精密度以及准确度均符合方法学验证的要求。分别在生品中检出16个共有峰,在炮制品检出8个共有峰;与对照样品(加纳的生品)相比,中国的两个样本相似系数分别为0.577(南京)和0.565(武汉)。结论:所建立的草决明指纹图谱的方法稳定可靠,适用于草决明质量评价,两个国家的草决明质量有明显差异。
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关 键 词: | 蒽 醌 草决明 指纹图谱 加 纳 HPLC |
Determination of Five Anthraquinones and Establishment of a Fingerprint of Cassia Occidentalis Seeds by HPLC with UV Detection |
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Institution: | HE Yongzhi, YAN Zhixue, Emestina Amponsem, et al ( Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ) |
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Abstract: | Objectiev : To establish a simultaneous determination of five cassia anthraquinones content by HPLC , and HPLC fingerprint method of grass cassia .M ethod:Hypersil C18 column (4.6 mm ×250 mm, 5μm), acetonitrile -0.05%formic acid as a mobile phase .The flow rate was 1.0 mL/min, the temperature was 30℃, and the detection wave length was 254 nm.Using chromatographic fingerprint evaluation software ( 2004 A) for data analysis , from Ghana and China , established HPLC fingerprint and similarity calculation .Result: The sensitivity, linearity , precision and accuracy of the method are in line with the requirements of method validation .16 and 8 common peaks were detected in crude drug and processed one respec-tively,compared with the control sample ( Ghana raw goods ) , similar coefficients of two samples from China were 0.577 ( Nanjing ) and 0.565 ( Wuhan ) , indicating significant difference between two areas of grass Cassia in quality .Conclusion:Cassia HPLC fingerprint method established stable and reliable , and suitable for quality evaluation Cassia herbs . |
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Keywords: | Anthraquinones Cassia occidentalis Fingerprint Ghana HPLC |
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