| 携带小鼠noggin基因的重组腺病毒载体对人骨髓间充质干细胞的神经样诱导作用 |
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| 引用本文： | 陶玉香,徐海伟,阴正勤.携带小鼠noggin基因的重组腺病毒载体对人骨髓间充质干细胞的神经样诱导作用[J].第三军医大学学报,2010,32(9). |
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| 作者姓名： | 陶玉香 徐海伟 阴正勤 |
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| 作者单位： | 1. 第三军医大学西南医院全军眼科中心, 重庆,400038
2. 400038 重庆,第三军医大学基础医学部生理学教研室,重庆市神经科学研究所 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的观察noggin基因能否单独将人骨髓间充质干细胞诱导为神经细胞以成为挽救神经退行性病变的"种子细胞"。方法原代培养人骨髓间充质干细胞,分为对照组、空白载体转染的Ad组和含有noggin基因的腺病毒载体转染的Ad-noggin组,观察转染后48h、4、7、10d等时相点各组细胞的形态学变化并行统计学分析。结果2种载体皆可成功转染人骨髓间充质干细胞使其自发绿色荧光,但Ad组形态无显著变化,而Ad-noggin组细胞在转染后48h即可见少量细胞分化为神经样细胞,转染后4d可见神经样细胞数目增多且伸出少量神经细胞样突起,10d时分化的细胞明显增多。与转染后7d相比,Ad-noggin组转染10d时胞体直经和树突直径明显增加(P0.05),但形态无改变。结论单独以含noggin基因的腺病毒载体转染人骨髓间充质干细胞可以使其向神经样细胞分化。
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| 关 键 词： | 间充质干细胞 分化 腺病毒 |
Noggin induces human bone marrow-derived mesenchymal stem cells to differentiate into neural cells |
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| Tao Yuxiang,Xu Haiwei,Yin Zhengqin.Noggin induces human bone marrow-derived mesenchymal stem cells to differentiate into neural cells[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(9). |
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| Authors: | Tao Yuxiang Xu Haiwei Yin Zhengqin |
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| Institution: | Tao Yuxiang1,Xu Haiwei2,Yin Zhengqin1 (1Ophthalmology Research Center,Southwest Hospital,2Department of Physiology,College of Basic Medical Sciences,Third Military Medical University,Chongqing,400038,China) |
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| Abstract: | Objective To study whether noggin alone can induce human bone marrow mesenchymal stem cells (MSCs) to differentiate into neural cells for the treatment of neural degenerative diseases.Methods Human bone marrow MSCs were primarily cultured and then divided into control,Ad group of empty adenovirus vector and Ad-noggin group of adenovirus vector with noggin gene.Their morphological changes were observed with fluorescence microscopy and laser confocal microscopy,and the results of 48 h,4,7,10 d were analyzed w... |
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| Keywords: | noggin |
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