| 用地高辛配基标记DNA探针进行菌落原位杂交 |
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| 引用本文： | 蒋三亮 张思仲. 用地高辛配基标记DNA探针进行菌落原位杂交[J]. 华西医科大学学报, 1992, 23(3): 245-247 |
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| 作者姓名： | 蒋三亮 张思仲 |
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| 摘 要： |
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| 关 键 词： | 地高辛配基 DNA探针 菌落原位杂交 |
Colony hybridization with digoxigenin labelled DNA probe] |
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| S Jiang,S Zhang. Colony hybridization with digoxigenin labelled DNA probe][J]. Journal of West China University of Medical Sciences, 1992, 23(3): 245-247 |
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| Authors: | S Jiang S Zhang |
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| Abstract: | A simple, rapid method for colony hybridization has been developed. The DNA probes were labelled by digoxigenin. Hybridization signal was detected by enzyme-linked immunoassay reaction. The results showed that this method is sensitive and reproducible, and it can be used for colony hybridization instead of isotopic methods. |
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