忍冬藤质量标准研究

 目的建立RP-HPLC测定忍冬藤中绿原酸、咖啡酸、当药苷和马钱子苷含量的方法,并以此对忍冬藤进行质量控制。方法色谱柱为Aglient Extend-C_{18(4.6 mm×250 mm,5μm);流动相为甲醇-0.4%醋酸水溶液(26∶74);流速1.0 mL·min^-1,检测波长240 nm。结果绿原酸、咖啡酸、当药苷和马钱子苷的线性范围和相关系数分别为23.5～470 mg·L^-1,r=0.999 9;12～240 mg·L^-1,r=0.999 7;10.5～210 mg·L^-1,r=0.999 9;13～260 mg·L^-1,r=0.999 8。平均回收率分别为97.7%(RSD=2.23%),96.6%(2.29%),95.0%(2.26%),103.3%(2.93%)。结论本方法简单、快速、可靠,可用于忍冬藤药材的质量控制。}

Studies on the Quality Control of Caulis Lonicerae Japonicae

OBJECTIVE To establish a RP-HPLC method for simultaneous determination of four bioactive compounds in Caulis Lonicerae Japonicae,i.e.chlorogenic acid,caffeic acid,sweroside and loganin.METHODS The separation was performed on an Aglient Extend-C₁₈ column(4.6 mm×250 mm,5 μm),using a isocratic elution with methanol-water(containing 0.4% acetic acid) as the mobile phase.The flow rate was 1.0 mL·min^-1,the detection wavelength was set at 240 nm.RESULTS The linear ranges and regression equations of chlorogenic acid,caffeic acid,sweroside and loganin were 23.5-470 mg·L^-1 with r=0.999 9;12-240 mg·L^-1 with r=0.999 7;10.5-210 mg·L^-1 with r =0.999 9 and 13-260 mg·L^-1 with r=0.999 8,respectively.The average recoveries(n=6) of the four constituents were 97.7%(RSD=2.23%),96.6%(2.29%),95.0%(2.26%),103.3%(2.93%),respectively.CONCLUSION This method is simple,rapid and reliable for the quality control of Caulis Lonicerae Japonicae.