三苯氧胺对胶质瘤C6细胞垂体瘤转化基因表达及肿瘤生长影响的体内研究

目的 探讨体内三苯氧胺对胶质瘤C6细胞垂体瘤转化基因(PTTG)表达及细胞生长的影响.方法 建立SD大鼠C6胶质瘤移植动物模型,将32只荷瘤裸鼠随机分为4组:空白对照组、高(2 mg·kg-1·d-1)、中(0.2 mg·kg-1·d-1)、低(0.02 mg·kg-1·d-1)剂量三苯氧胺作用组,共给药20 d.空白对照组给等量的生理盐水.定期观察肿瘤生长情况,测量肿瘤体积,计算抑瘤率.处死全部动物模型,剔出肿瘤,逆转录-聚合酶链反应(RT-PCR)检测FITG mRNA表达.结果 与空白对照组相比,各组三苯氧胺均能抑制肿瘤生长,其体积抑瘤率分别为47.6%、35.5%、21.2%,各组间差异均有统计学意义(P<0.05);FITG mRNA的表达在低、中、高剂量组均降低,各组间差异均有统计学意义(P<0.05).结论 三苯氧胺能以量效的方式抑制胶质瘤PTTG的表达和肿瘤生长,本研究为临床应用三苯氧胺治疗胶质瘤提供了理论基础.

Effect of tamoxifen on pituitary tumor transforming gene expression in C6 cell and tumor growth of glioma in vivo

Objective To investigate the effect of tamoxifen on pituitary tumor transforming gene ex-pression in C6 cell and tumor growth of glioma in vivo. Methods Animal models were established on 32 SD rats with C6 cells of glioma. The rats bearing with C6 glioma were divided into 4 groups randomly, which were treated without tamoxifen or with different doses of tamoxifen(0. 02 mg · kg-1 · d-1, 0. 2 mg · kg-1 · d-1, 2mg · kg-1 · d-1) once a day for 20 days. The dimension of tumors were measured, the tumor inhibition rates were caculated, and living state of the rats were observed. The expression of PTTG mRNA was detected by RTPCR. Results All kinds of doses of tamoxifen could inhibit the tumors growth in rats with C6 glioma, and the tumor volume were reduced by 47.6%, 35.5% and 21.2% in the high-, middle-and low-dose groups respec-tively, there were significantly differences among the 4 groups ( P < 0. 05 ). Low-, middle- and high- dose of tamoxifen all could inhibit the expression of PTTG mRNA, and there were significantly differences among the 4 groups in PTTG mRNA expression ( P < 0. 05 ). Conclusion Tamoxifen can inhibit PTTG expression and the growth of glioma cell line C6 in vivo significantly in dose-dependent manner, which provides a theory basis for clicinal use of tamoxifen in patients with gliomas.