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| 树舌多糖GF抑制小鼠HepA瘤生长机制的初步研究 | |
| 引用本文： | 王玉,王璐,于英君,夏春辉. 树舌多糖GF抑制小鼠HepA瘤生长机制的初步研究[J]. 中国现代医学杂志, 2008, 18(21): 3083-3086 |
| 作者姓名： | 王玉 王璐 于英君 夏春辉 |
| 作者单位： | 1. 齐齐哈尔医学院,生物遗传教研室,黑龙江,齐齐哈尔,161042 2. 国际遗传学杂志编辑部,黑龙江,哈尔滨,150086 3. 黑龙江中医药大学,生物化学与分子生物学教研室,黑龙江,哈尔滨,150040 |
| 基金项目： | 黑龙江省自然基金(No:D2007-114); 黑龙江省普通高等学校骨干教师创新能力资助计划(No:1054G070和No:1055G066); 黑龙江省教育厅资助课题(No:10511129) |
| 摘 要： | 目的 该研究主要初步探讨树舌多糖(Ganoderm applanatum polysaccharide,GAP)GF抑制小鼠HepA瘤的生长机制.方法 树舌多糖GF注射到HepA小鼠后,通过测定瘤体的质量计算树舌多糖抑瘤率,通过电镜观察HepA细胞的超微结构,通过TUNEL法检测凋亡细胞,通过Western-blot检测Fas、caspase-3、Bcl-2的表达.结果 树舌多糖GF注射到HepA小鼠后,GAPGF的抑瘤率为51.82%;电镜下观察到HepA细胞核固缩、染色质浓缩及凋亡小体形成等典型的细胞凋亡特征;TUNEL分析显示GAPGF能明显诱导HepA细胞凋亡;Western blot分析显示GAPGF可使Fas与cmpase-3表达上调,Bcl-2表达降低.结论 GAPGF通过诱导HepA细胞凋亡抑制小鼠HepA瘤的生长,其分子机制可能与Fas的激活、caspase-3表达的上调以及Bcl-2表达的降低有关. |
| 关 键 词： | 树舌多糖GF 凋亡 fas caspase-3 Bcl-2 |
Primary mechanism of ganoderm applanatum polysaccharide GF suppressing HepA tumor in mouse |
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| WANG Yu , WANG Lu , YU Ying-Jun , XIA Chun-Hui. Primary mechanism of ganoderm applanatum polysaccharide GF suppressing HepA tumor in mouse[J]. China Journal of Modern Medicine, 2008, 18(21): 3083-3086 | |
| Authors: | WANG Yu WANG Lu YU Ying-Jun XIA Chun-Hui |
| Affiliation: | WANG Yu1,WANG Lu2,YU Ying-Jun3,XIA Chun-Hui1(1.Department of Biology , Genetics,Qiqihaer Medical College,Qiqihaer,Heilongjiang,161042,P.R.China,2.Editorial office of International Journal of Genetics,Harbin,150086,3.Department of Biochemistry , Molecular Biology,Heilongjiang Traditional Medical University,150040,P.R.China) |
| Abstract: | Objective To investigate the primary mechanism of Ganoderm Applanatum Polysaccharide(GAP) GF suppressing HepA tumor in mouse.MethodsAfter HepA mouse was injected with GAPGF, the inhibition rate of GAPGF on tumor growth was determined by measuring tumor body mass, the ultrastructure of HepA cells was observed under an electron microscopy, the apoptotic cells were detected by TUNEL method, and the expression levels of Fas, Caspase-3 and Bcl-2 were measured by Western-blot assay.ResultsAfter HepA mouse was inj... |
| Keywords: | ganoderm applanatum polysaccharide GF apoptosis fas caspase-3 Bcl-2 |
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