| 大蒜素诱导THP-1细胞凋亡的作用及机制 |
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| 引用本文： | 廖〓洋,陈建斌,卢前微,葛群芳,杨泽松. 大蒜素诱导THP-1细胞凋亡的作用及机制[J]. 南京医科大学学报(自然科学版), 2010, 0(2): 155-160 |
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| 作者姓名： | 廖〓洋 陈建斌 卢前微 葛群芳 杨泽松 |
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| 作者单位： | 重庆医科大学附属第一医院血液内科; |
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| 基金项目： | 重庆市卫生局医学科学技术研究项目基金资助(07-2-035) |
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| 摘 要： | 目的:探讨大蒜素对急性单核细胞白血病细胞株THP-1凋亡及凋亡基因Fas/FasL表达的影响。方法:采用四甲基偶氮唑蓝(MTT)法测定大蒜素对THP-1细胞增殖抑制率;AO/EB染色观察细胞凋亡形态;Annexin V-FITC/PI双染法流式细胞术(FCM)检测凋亡率的变化;FCM检测细胞凋亡率和细胞周期分布;免疫细胞化学法和Western blot观察细胞Fas、FasL蛋白表达。结果:MTT显示大蒜素能抑制THP-1细胞增殖,呈时间-剂量依赖性,72h中效浓度(IC50)为12.8mg/L。Annexin V-FITC/PI检测凋亡率随大蒜素浓度增加而增加。6.4、12.8mg/L大蒜素作用THP-1细胞72h后,FCM检测G0/G1期细胞比率明显上升,而S期比率明显下降,均出现凋亡峰,凋亡率分别为(22.93±2.93)%和(36.73±2.88)%,呈剂量依赖性,与对照组比较有显著差异(P<0.01)。在荧光显微镜下可区分早、晚期凋亡细胞、活细胞和坏死细胞。免疫组化显示大蒜素作用后Fas/FasL蛋白上调,主要分布在细胞浆和细胞膜。Western blot结果显示Fas/FasL蛋白表达量随大蒜素浓...
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| 关 键 词： | 大蒜素 THP-1细胞 凋亡 细胞周期 Fas/FasL |
Allicin induced apoptosis of THP-1cells and its mechanism |
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| LIAO Yang,CHEN Jian-bin,LU Qian-wei,GE Qun-fang and YANG Ze-song. Allicin induced apoptosis of THP-1cells and its mechanism[J]. Acta Universitatis Medicinalis Nanjing, 2010, 0(2): 155-160 |
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| Authors: | LIAO Yang CHEN Jian-bin LU Qian-wei GE Qun-fang YANG Ze-song |
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| Affiliation: | Department of Hematology/a>;First Affiliated Hospital of Chongqing Medical University/a>;Chongqing 400016/a>;China |
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| Abstract: | Objective:The purpose of this paper is to investigate the effects of allicin on the apoptosis of THP-1 cells and expressions of Fas / FasL. Methods:The proliferation inhibition rates of THP-1 cells after various treatments were examined by MTT assay. After the treatment of THP-1 cells by allicin,morphological change of apoptotic cells was investigated by AO / EB fluorescent staining under fluorescent microscope. Apoptosis rate was determined with Annexin V-FITC / PI double staining by flow cytometry. Flow c... |
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| Keywords: | allicin THP-1 cells apoptosis cell cycle Fas / FasL |
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