终末期肾病中DNA氧化损伤标志物8-OHdG水平的研究

目的通过检测终末期肾病（ESRD）患者血清中的DNA氧化损伤标志物8-羟基脱氧鸟苷（8-OHdG）含量,探讨ESRD患者中DNA氧化损伤的程度及其影响因素。方法选取年龄及性别匹配的研究对象40例,分为3组：对照组、CRF（慢性肾功能不全）组和HD（血液透析）组。采用ELISA法检测血清8-OHdG水平,改良镀铜镉颗粒还原法检测一氧化氮（NO）水平,硫代巴比妥酸产物比色法检测丙二醛（MDA）水平。结果与对照组相比,CRF组、HD组透析前、后血清8-OHdG水平均显著增高（P〈0.01）,且HD组透析后8-OHdG水平较透析前显著增高（P〈0.05）。CRF组、HD组透析前和透析后NO、MDA水平较对照组明显增高（P〈0.05）。血清8-OHdG和Scr、NO水平呈显著正相关（P〈0.05）。结论在ESRD患者血清中8-OHdG水平明显增高,提示DNA氧化损伤增强。8-OHdG作为一种DNA氧化损伤产物,可被认为是评价ESRD氧化损伤水平的可靠观测指标。

Study of serum 8-hydroxy-2''''-deoxyguanosine level in patients with end stage renal diseases

Objective A growing body of evidence suggests that oxidative stress is enhanced in patients with end stage renal diseases （ESRD） and is involved in pathogenesis of related chronic complications. Reactive oxygen species （ROS） may cause DNA damage and increase of cancer mortality in ESRD patients. But the detailed mechanism still remains unclear, and the degree of DNA oxidative damage in ESRD patients has not been well examined. We measured serum 8-hydroxy-2＇-deoxyguanosine （8-OHdG）, a product derived from interaction of oxygen radicals with deoxyguanosine, as well as nitric oxide （NO） and malondialdehyde （MDA） in patients with ESRD to investigate the degree of DNA oxidative damage and its related factors in ESRD. Methods Forty subjects were enrolled in this study, in which 15 subjects were non-dialysis ESRD patients （CRF group）, 15 were ESRD patients undergoing regular hemodialysis （HD group）, and 10 age-and sex-matched health people were selected as controls. Patients in this study had no inflammatory or malignant diseases, and no supplement with vitamin C, E and iron ion. Smokers and patients with positive hepatitis C virus antibody or hepatitis B surface antigen were excluded. Serum 8-OHdG was measured by an enzyme-linked immunosorbent assay （ELISA） kit, NO was measured by a modified cadmium particle reduction method, and MDA was measured by a thiobarbital product chromatometry method. Results Serum 8-OHdG was significantly higher in CRF group and HD group before and after hemodialysis than in normal subjects （P〈 0.01）. In HD patients, serum 8-OHdG was increased after hemodialysis, as compared with that before hemodialysis （P〈0.05）. In addition, serum NO and MDA were also significantly higher in CRF group and HD group before and after hemodialysis than in normal subjects （P 〈 0.05）. Serum 8-OHdG had a significant positive correlation with serum creatinine （r = 0.368, P 〈 0.05） and NO （r = 0.453, P 〈 0.01）, but had an insignificant correlation with