蝎毒抗癌多肽对放疗后肝癌小鼠瘤重和一般状况影响的初步观察

目的　观察蝎毒抗癌多肽 (APBMV)与放疗 (RT)合用对肝癌小鼠的作用。方法　取H2 2 带瘤小鼠 10 0只 ,采用肿瘤生长抑制率 (IR)、白细胞计数和血红蛋白含量、SOD活力、LPO含量测定及脾重指数 (SI)等指标 ,观察不同剂量的APBMV与放疗合用后上述指标的变化。结果　放疗后 6d及 9d ,RT与APBMV合用后瘤重明显降低 ,IR最高达 78 2 9%与 70 45 % (与RT组和APB MV组比较 ,P <0 0 5 ,或P <0 0 1) ,白细胞计数和SI较RT组显著升高 (P <0 0 5 ,P <0 0 1) ;血红蛋白含量测定结果无明显差异 ;RT组SOD活力最低 ,LPO含量最高 (与对照组比较P <0 0 5 ,P <0 0 1) ,RT APBMV高剂量组SOD活力明显提高 ,LPO含量显著降低 (P <0 0 1) ,接近空白对照水平。结论　APBMV与放疗合用对H2 2 的抑制作用比单纯放疗和单用APBMV增强 ,APBMV并能减轻辐射对带瘤小鼠机体的损伤。

Primary observation on the effect of APBMV on tumor weight and general physical condition of hepatoma 22-bearing mice after radiotherapy

Objective\ To observe the effect of antineoplastic polypeptide from Buthus martensii venom (APBMV) combined with radiotherapy on hepatoma-bearing mouse. Methods \ Hundreds H 22 -bearing mice were used in this experiment.The tumor growth inhibiting rate (IR%),WBC count,hemoglobin content,activity of superoxide dismutase (SOD),level of lipid peroxide (LPO) and spleens index (SI) were used as the parameters.After radiotherapy (RT) or after administration of different dosage of APBMV combined with RT,the changes of these parameters were observed. Results\ On the 6th and 9th day after radiotherapy,the tumor weights decreased after administrating APBMV combined with RT,in which IR were 78 29% and 70 45%,respectively (comparing with RT and APBMV group, P <0 05 or P <0 01).In RT APBMV group,WBC count and SI were also higher than those of the RT group ( P <0 05, P <0 01 ).The results of measurement of hemoglobin content in peripheral blood indicated that there were no differences ( P >0 05) among all groups.SOD activity was the lowest and LPO level was the highest in RT group (comparing with the control group, P <0 05, P <0 01).Combining the high dosage of APBMV with RT to treat H 22 -bearing mice,SOD activity increased and LPO level decreased evidently (comparing with RT group, P <0 01)and approached the levels of the control group. Conclusion \ The inhibiting effect of the combining therapy (APBMV combining with RT)on H 22 was stronger than radiation or APBMV alone.APBMV can also antagonize radiation injury on H 22 -bearing mice.