| 6A8 cDNA编码一种α-甘露糖苷酶 |
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| 引用本文： | Shi G,Ma F,Zhu L,Zhang L,Zhao F. 6A8 cDNA编码一种α-甘露糖苷酶[J]. 中国医学科学院学报, 1999, 21(6): 439-443 |
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| 作者姓名： | Shi G Ma F Zhu L Zhang L Zhao F |
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| 作者单位： | 中国医学科学院中国协和医科大学基础医学研究所免疫学教研室,北京,100005 |
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| 基金项目： | 国家自然科学基金!(3 960 3 0 3 0 0 ) |
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| 摘 要： | 目的 探讨６Ａ８ ｃＤＮＡ编码的蛋白质的性质。方法 依据Ｃｏｎ Ａ的配体是基露糖,而α－甘露糖苷酶的作用是剪细胞糖蛋白聚糖中苷露糖的原理,采用细胞免疫荧光染色法以及激光共聚焦显微镜观察经转染６Ａ８ｃＤＮＡ正义或反义重组表达载体后,细胞结合ＣｏｎＡ的强弱,验证６Ａ８ｃＤＮＡ编码的蛋白质是否为一种α－甘露－糖苷酶。结果 ＣＯＳ－６ 人鼻咽癌细胞株ＣＮＥ－２Ｌ２转染ｐＲｃ／ＣＭＶ－正交６Ａ８ｃＤＮＡ后与
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| 关 键 词： | 6A8cDNA α－甘露糖苷酶 ConA结合 |
| 修稿时间： | 1998-07-15 |
6A8 cDNA encodes A type of alpha-mannosidase |
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| Shi G,Ma F,Zhu L,Zhang L,Zhao F. 6A8 cDNA encodes A type of alpha-mannosidase[J]. Acta Academiae Medicinae Sinicae, 1999, 21(6): 439-443 |
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| Authors: | Shi G Ma F Zhu L Zhang L Zhao F |
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| Affiliation: | Department of Immunology, Insitutute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005. |
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| Abstract: | OBJECTIVE: To find the nature of the protein encoded by 6A8 cDNA. METHODS: In the light of that the ligand of Con A is mannoses and that the function of alpha-mannosidase is trimming of mannoses in the oligosaccharide chains of glycoproteins, COS-7 cells and CNE-2L2 cells, a cloned cell line of human nasopharyngeal carcinoma, were transfected with pRc/CMV-sense 6A8 cDNA or pRc/CMV-antisense 6A8 cDNA and the Con A binding to the cells was examined upon a lazer scan confocal microscope. RESULTS: The data demonstrated that with transfection of pRc/CMV-sense 6A8 cDNA the binding of Con A to the cells was decreased, but the staining of the cells with mAb 6A8 was enhanced, and vise versa for the cells with transfection of pRc/CMV-antisense 6A8 cDNA. CONCLUSIONS: The protein encoded by 6A8 cDNA might be a type of alpha-mannosidase recognized by mAb 6A8. |
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