6A8 cDNA编码一种α-甘露糖苷酶

目的 探讨６Ａ８ ｃＤＮＡ编码的蛋白质的性质。方法 依据Ｃｏｎ Ａ的配体是基露糖,而α－甘露糖苷酶的作用是剪细胞糖蛋白聚糖中苷露糖的原理,采用细胞免疫荧光染色法以及激光共聚焦显微镜观察经转染６Ａ８ｃＤＮＡ正义或反义重组表达载体后,细胞结合ＣｏｎＡ的强弱,验证６Ａ８ｃＤＮＡ编码的蛋白质是否为一种α－甘露－糖苷酶。结果 ＣＯＳ－６ 人鼻咽癌细胞株ＣＮＥ－２Ｌ２转染ｐＲｃ／ＣＭＶ－正交６Ａ８ｃＤＮＡ后与

6A8 cDNA encodes A type of alpha-mannosidase

OBJECTIVE: To find the nature of the protein encoded by 6A8 cDNA. METHODS: In the light of that the ligand of Con A is mannoses and that the function of alpha-mannosidase is trimming of mannoses in the oligosaccharide chains of glycoproteins, COS-7 cells and CNE-2L2 cells, a cloned cell line of human nasopharyngeal carcinoma, were transfected with pRc/CMV-sense 6A8 cDNA or pRc/CMV-antisense 6A8 cDNA and the Con A binding to the cells was examined upon a lazer scan confocal microscope. RESULTS: The data demonstrated that with transfection of pRc/CMV-sense 6A8 cDNA the binding of Con A to the cells was decreased, but the staining of the cells with mAb 6A8 was enhanced, and vise versa for the cells with transfection of pRc/CMV-antisense 6A8 cDNA. CONCLUSIONS: The protein encoded by 6A8 cDNA might be a type of alpha-mannosidase recognized by mAb 6A8.