| 一种膜荚黄芪凝集素的分离纯化研究 |
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| 引用本文： | 朱利芬,闫巧娟,江正强,KumarNarasimh,黄林华.一种膜荚黄芪凝集素的分离纯化研究[J].中草药,2010,41(5):714-717. |
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| 作者姓名： | 朱利芬 闫巧娟 江正强 KumarNarasimh 黄林华 |
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| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083
2. 中国农业大学工学院,北京,100083 |
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| 基金项目： | 国家自然科学基金,教育部新世纪优秀人才支持计划 |
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| 摘 要： | 目的从膜荚黄芪根中分离纯化一种凝集素。方法通过硫酸铵分级沉淀和ConA-Sepharose4B亲和色谱从膜荚黄芪根浸提液中分离纯化一种凝集素。结果经过上述两步纯化得到电泳纯的凝集素,SDS-PAGE和Superdex75凝胶过滤色谱测定该凝集素的相对分子质量分别为3.15×104和3.35×104,糖蛋白染色显示其为糖蛋白,总糖的量为10.7%。结论从膜荚黄芪根中分离纯化得到一种凝集素,该蛋白为单亚基糖蛋白,具有对兔血红细胞的凝集活性,凝集活性的比活力为391.9U/mg。
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| 关 键 词： | 膜荚黄芪 凝集素 纯化 亲和色谱 |
| 收稿时间： | 2009/8/19 0:00:00 |
Isolation and purification of a lectin from roots of Astragalus membranaceus |
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| ZHU Li-fen,YAN Qiao-juan,JIANG Zheng-qiang,KUMAR Narasimha and HUANG Lin-hua.Isolation and purification of a lectin from roots of Astragalus membranaceus[J].Chinese Traditional and Herbal Drugs,2010,41(5):714-717. |
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| Authors: | ZHU Li-fen YAN Qiao-juan JIANG Zheng-qiang KUMAR Narasimha and HUANG Lin-hua |
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| Institution: | College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;College of Engineering, China Agricultural University, Beijing 100083, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;College of Engineering, China Agricultural University, Beijing 100083, China |
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| Abstract: | |
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| Keywords: | Astragalus membranaceus(Fisch )Bunge|lectin|purification|affinity chromatography |
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