褐藻多糖对氧化应激诱导的淋巴细胞DNA损伤的保护作用

研究褐藻多糖（ＢＳＰ）对活性氧引起的淋巴细胞ＤＮＡ损伤的保护作用。方法是敏感的单细胞凝胶电泳（ＳＣＧＥ）。结果表明在不同的介质中Ｈ_２Ｏ_２对细胞ＤＮＡ的损伤程度不同，在同一介质中Ｈ_２Ｏ_２对细胞ＤＮＡ的损伤程度呈一定的量效关系。 １００ｍｇ／Ｌ和 ２００ｍｇ／Ｌ的大分子量多糖（ＢＳＰｈ）和小分子量多糖（ＢＳＰｓ）都能明显降低Ｈ_２Ｏ_２对细胞ＤＮＡ损伤程度。表明ＢＳＰ能够清除自由基，减低氧化应激所致的细胞ＤＮＡ损伤程度，有利于维护细胞的正常结构和功能。提示 ＢＳＰ可作为一种预防性抗氧化营养素，用于预防氧化应激引起的疾病。

THE PROTECTIVE EFFECT OF THE SULFATED POLYSACCHARIDES FROM BROWN SEAWEED ON LYMPHOCYTE DNA DAMAGE CAUSED BY OXYGENRADIAL

The purpose of this study is to investigate the protective effects of sulfated polysaccharides (BSP)from brown seaweeds (large molecule BSPh and small molecule BSPs ) on oxygen-radial-generated DNA damage in human lymphocytes. A sensitive method-single cell gelelectrophoresis (SCGE)was used to observe the lymphocytes DNA damage. The re sults showed that in different mediums the DNA damage rate of human lymphocytes treated with H_2O_2 was different,but in the same medium the DNA damage grade of human lympho cytes had dose-effect relationships. BSPh and BSPs (100mg/L and 200mg/L)showed the pro tective effects,respectively. These results suggested that BSP might have the scavenging ef fects on radial produced by H_2O_2,and reduce the oxygen-radial-generated DNA damage in human lymphocytes,maintain normal structure and function of cells and be beneficial in pre vention for some diseases associated with oxidative stress.