|
|||||
|
|
Molecular discrimination of sheep bovine spongiform encephalopathy from scrapie |
|
| Authors: | Pirisinu Laura Migliore Sergio Di Bari Michele Angelo Esposito Elena Baron Thierry D'Agostino Claudia De Grossi Luigi Vaccari Gabriele Agrimi Umberto Nonno Romolo |
| Affiliation: | Author affiliations: Istituto Superiore di Sanità, Rome, Italy (L. Pirisinu, S. Migliore, M.A. Di Bari, E. Esposito, C. D’Agostino, G. Vaccari, U. Agrimi, R. Nonno);;Agence Nationale de Sécurité Sanitaire, Lyon, France (T. Baron);;Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Rome (L. De Grossi) |
| Abstract: | Sheep CH1641-like transmissible spongiform encephalopathy isolates have shown molecular similarities to bovine spongiform encephalopathy (BSE) isolates. We report that the prion protein PrPSc from sheep BSE is extremely resistant to denaturation. This feature, combined with the N-terminal PrPSc cleavage, allowed differentiation of classical scrapie, including CH1641-like, from natural goat BSE and experimental sheep BSE. |
| Keywords: | Prions transmissible spongiform encephalopathy scrapie bovine spongiform encephalopathy BSE CH1641 sheep Western blot dispatch |
| 本文献已被 PubMed 等数据库收录！ | |