| 蛇床子素预处理对兔心肌缺血／再灌注损伤的保护作用研究 |
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| 引用本文： | 马同强,梁智星.蛇床子素预处理对兔心肌缺血／再灌注损伤的保护作用研究[J].山西职工医学院学报,2008,18(2):6-8. |
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| 作者姓名： | 马同强 梁智星 |
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| 作者单位： | 山西医科大学第一医院,山西,太原,030001 |
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| 摘 要： | 目的：探讨蛇床子素预处理对兔急性心肌缺血／再灌注损伤的保护作用及其机制。方法：结扎新西兰大耳兔左冠状动脉前降支40min后，松开结扎线再灌注120min，制作急性心肌缺彬再灌注损伤模型。缺血／再灌注（I／R）组：缺．血40min，放松结扎线后再灌注120min；缺血预处理（IPC）组：缺血5min，再灌注5min，反复3次，余处理方法同I／R组；蛇床子素预处理（Ost）组：缺血前30min经耳缘静脉静注蛇床子素25mg／kg，5min内注完，余处理方法如I／R组。于实验结束时从右心室取血测定血清超氧化物歧化酶（SOD）、丙二醛（MDA）含量及心肌肌钙蛋白（cTnI）、乳酸脱氢酶同工酶（LDH）、肌酸激酶（CK）、肌酸激酶同功酶（CK—MB）漏出率；采用TUNEL法原位标记缺血区凋亡心肌细胞并计算凋亡率。结果：各实验组血清SOD活性均较对照组高，MDA含量、cTnI及心肌酶（LDH、CK及CK—MB）漏出率均较对照组低（P〈0．05）；各实验组心肌细胞凋亡率均低于对照组（P〈0．05）。结论：蛇床子素注射液预处理可减轻兔急性心肌缺血／再灌注损伤、减少心肌细胞凋亡，对心肌损伤可能有保护作用。
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| 关 键 词： | 蛇床子素 心肌缺血/再灌注 心肌保护 机制 |
Study on the Protective Effect of Osthole Preconditioning for Myocardial Ischemia-Reperfusion Injury in Rabbits |
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| MA Tongqiang,LIANG Zhixing.Study on the Protective Effect of Osthole Preconditioning for Myocardial Ischemia-Reperfusion Injury in Rabbits[J].Journal of Shanxi Medical College For Continuing Education,2008,18(2):6-8. |
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| Authors: | MA Tongqiang LIANG Zhixing |
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| Institution: | MA Tongqiang LIANG Zhixing (The First Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi,China) |
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| Abstract: | Objective: To study the protective effect and mechanism of osthole preconditioning to acute myocardial ischemia-reperfusion injury in rabbits.Methods: The model of acute myocardial ischemia-reperfusion injury was made after the left anterior descending branch of coronary artery of a New Zealand rabbit was occluded for 40 min and then reperfused with loosing the ligature for 120 min.Ischemia-reperfusion(I/R)group was occluded ischemia for 40 min and then reperfused for 120 min after loosing the ligature.Isch... |
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| Keywords: | osthole myocardial ischemia and reperfusion myocardial protection mechanism |
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