| HPLC法测定射干抗病毒注射液中绿原酸和咖啡酸的含量 |
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| 引用本文： | 索银科,罗兰.HPLC法测定射干抗病毒注射液中绿原酸和咖啡酸的含量[J].中国药事,2010,24(4):386-388. |
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| 作者姓名： | 索银科 罗兰 |
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| 作者单位： | 1. 陕西省宝鸡市药品检验所,宝鸡,721000
2. 宝鸡市食品药品监督管理局 |
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| 摘 要： | 目的建立HPLC法测定射干抗病毒注射液中绿原酸和咖啡酸的含量。方法采用Hypersil ODS柱,0.01mol·L^-1磷酸二氢钠溶液（1%磷酸溶液调节pH值至3.9）-甲醇（85∶15）为流动相,流速：1.0mL·min^-1,检测波长：323nm,柱温：30℃。结果绿原酸和咖啡酸浓度分别在0.40-200mg·L^-1（r=0.9996）、0.16-160mg.L-1（r=0.9986）范围内与峰面积呈良好线性关系,平均加样回收率分别为99.8%,100.7%。结论本法简便、准确、重复性好,可用于射干抗病毒注射液中绿原酸和咖啡酸含量的同时测定。
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| 关 键 词： | HPLC 射干抗病毒注射液 绿原酸 咖啡酸 |
Determination of Chlorogenic Acid and Caffeic Acid in Shegan Kangbingdu Injection by HPLC |
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| Suo Yinke , Luo Lan.Determination of Chlorogenic Acid and Caffeic Acid in Shegan Kangbingdu Injection by HPLC[J].Chinese Pharmaceutical Affairs,2010,24(4):386-388. |
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| Authors: | Suo Yinke Luo Lan |
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| Institution: | Suo Yinke , Luo Lan1(Baoji Municipal Institute for Drug Control,Baoji 721001,1Baoji Municipal Food , Drug Administration) |
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| Abstract: | Objective A HPLC method for determination of chlorogenic acid and caffeic acid in Shegan Kangbingdu Injection was established.Methods A Hypersil ODS column was used with 0.01mol·L-1 sodium dihydrogen phosphate（adjust pH value to 3.9 with 1% phosphate acid solution） and methal（85∶15） as mobile phase at flow rate of 1.0mL·min^-1.The detection wavelength was 323nm and the column temperature was 30℃.Results The linear ranges of chlorogenic acid and caffeic acid were 0.40～200mg·L^-1（r=0.9996） and 0.16～160mg·L^-1（r=0.9986）,respectively.The average recoveries were 99.8% and 100.7%,respectively.Conclusion The method is simple,accurate and reproducible for determination of chlorogenic acid and caffeic acid in Shegan Kangbingdu Injection simutaneously. |
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| Keywords: | HPLC |
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