氯通道在一氧化氮诱导离体大鼠海马神经元凋亡中的作用

目的 观察氯通道阻断剂SITS和DIDS对NO诱导的大鼠离体海马神经元凋亡的效应，探讨氯通道在缺血性脑损伤中的作用。方法 离体培养12天的SD大鼠海马神经元，随机分为正常对照组、NO处理组、NO处理后使用氯通道阻断剂组，对各组神经元分别在相应的时间点进行Hoechst荧光染色观察凋亡细胞数和MTT实验定量检测神经元的存活率，western blot 分析凋亡信号分子caspase-3的变化。结果 SITS和DIDS呈剂量依赖性地抑制NO诱导的神经元损伤，并能抑制损伤所引起的caspase-3的激活，提高神经元的存活率。结论 氯通道阻断剂对NO诱导的大鼠海马神经元凋亡有一定的保护作用。

The role of chloride channel on the cultured hippocampal neuronal apoptosis induced by NO in rats

Objective To observe the effects of SITS and DIDS, two kinds of chloride channel blockers, on the hippocampal neuronal damage induced by NO in culture and to explore the roles of chlorides in the cerebral ischemic injury. Methods The cultures were divided into three groups, they were control group, NO treatment group, NO treatment plus chloride channel blocker group. The cultures were detected with the methods of morphological stain (Hoechst 33258) and MTT quantitative analysis to observe the apoptotic neurons and neuronal viabilities. The activated caspase-3 were analysed with western blot. Results There was a significant protective effects of SITS and DIDS on neuronal damage with dose-dependence. Conclusions Chloride channel blockers had some protective effects against neuronal injury induced by NO.