兔眼准分子激光角膜切削术联合羊膜移植术的初步实验研究

目的：以准人子角膜切削（PTK）术后的兔眼为模型，探讨保存人羊膜移植对角膜伤口早期愈合的影响，方法：新西兰西兔12只，双眼行PTK术，一眼术后立于角膜表面移植保存的人羊膜，术后，每日进行临床观察，并分别于第3，5，7天处死动物，取角膜行光镜和电镜检查，结果：在术后3，5，7天，羊膜移植侧角膜中央上皮增生厚度均明显小于对照后角膜上皮下雾状混浊（haze)的形成中发挥作用。

The Tentative Experimental Study of Phototherapeutic Keratotomy Combined with Amniotic Membrane Transplantation in Rabbit Corneal

Objective:To establish the model of phototherapeutic keratotomy(PTK) in rabbits, and to investigate the effect of preserved amniotic membrane transplantation on initial healing stage of corneal wound. Methods:Twenty four eyes of 12 New Zealand white rabbits were operated by PTK. Preserved human amniotic membrane transplantation was planted in one eye immediately, and the another eye was used as control. Clinical examination was performed every day after operation. The rabbits were separately killed after 3,5,7 days of operation, and their corneal were prepared for optical and electron microscopy examination. Results:The regeneration of corneal epithelium in amniotic membrane transplantation side was significantly less than the control after 3,5,7 days of operation. There was obvious difference between two eyes. Apoptosis of epithelium cells in control was more than in amniotic membrane transplantation. Conclusion:Amniotic membrane has the role of adjusting healing of corneal wound, and is an ideal material for corneal surface reconstruction, and could reduce the Haze post PTK.