人canstatin基因对人食管癌裸鼠移植瘤中bcl-xl和cyclin D1表达的影响及意义

目的探讨人canstatin基因对人食管癌裸鼠移植瘤的的影响作用,寻求其抑制肿瘤生长的机制。方法 KYSE150细胞建立裸鼠食管癌动物模型,Ad-canstatin实施干预,RT-PCR检测肿瘤组织bcl-xl、cy-clinD1的mRNA的表达水平。免疫组织化学方法检测微血管密度（MVD）。结果治疗组肿瘤体积明显小于两个对照组,差异有统计学意义（P〈0.05）,而两对照组间差异无统计学意义。对电泳结果进行灰度分析和统计学分析显示canstatin治疗组cyclinD1、bcl-xl的mRNA表达明显低于GFP组和PBS组,差异均有统计学意义（P〈0.05）。canstatin治疗组MVD明显低于对照组。结论人canstatin基因对人食管癌移植瘤的生长具有抑制作用,其作用机制可能与抑制cyclin D1、bcl-xl表达而抑制肿瘤血管生成有关。

Study on Effect and Significance of Canstatin Gene on Expressions of bcl-xl and Cyclin D1 on Human Esophageal Carcinoma Xenografts in Nude Mice

Objective This study was to investigate the antitumor effects of canstatin gene on human esophageal carcinoma xenografts in nude mice.Methods Esophageal cancer celles were implanted into BALB/c nude mice to induce tumor xenografts,tumor xenografts were randomized into three groups： PBS,adenovirus green fluorescent protein（Ad-GFP）,and Ad-GFP-canstatin groups.After treatment,tumor size was measured.The expression of cyclin D1、bcl-xl mRNA Ievels were detected by RT-PCR and microvessel density（MVD） in tissue of each group were detected by immunohistochemistry.Results Compared with that in Ad-GFP and PBS groups,tumor size in Ad-GFP-canstatin group was significantly suppressed after gene transfection.Compared with Ad-GFP and PBS groups,expressions of cyclinD1 and bcl-xl in Ad-GFP-canstatin group was lower（P0.05）,and microvessel density（MVD） were lower too（P0.05）.Conclusion Canstatin can inhibit the growth of human esophageal carcinoma by suppressing angiogenesis via down-regulating cyclin D1 and bcl-xl expression.