姜黄素纳米脂质载体中药物含量及包封率测定方法的建立

目的建立测定姜黄素纳米脂质载体中药物含量及包封率的方法。方法采用超滤离心法分离纳米粒与游离药物,采用高效液相法测定药物的含量及包封率。色谱柱:COSMILC18柱(4.6mm×250mm,5μm);检测波长:430nm;流动相:乙腈:4%冰乙酸(60:40);流速:1.0ml/min;柱温:30℃;进样量:20μl。结果姜黄素在0.050～40.0μg/ml范围内线性关系良好(r=0.9998,n=8)。采用离心超滤法测得的3批样品的包封率分别为98.11%,97.18%,98.59%。3批样品的载药量分别为3.89%,3.76%,4.12%。结论该方法专属性强、方便、快捷,适用于测定姜黄素纳米脂质载体中药物的含量及包封率。

Methodology for determination of contents and entrapment efficiency of curcumin nanostructured lipid carrier

Objective To establish a methodology for determination of the contents and entrapment efficiency of curcumin nanostructured lipid carrier. Methods Nanoparticles and free drugs were separated by ultrafiltration centrifugation for subsequent determination of the contents and entrapment efficiency of curcumin nanostructured lipid carrier by high-performance liquid chromatography （HPLC）. The HPLC system consisted of a reverse phase COS- MOSIL C18 column （4.6 min×250 mm, 5 μm） with a capacity for detection of UV at the wavelength of 430 nm. The mobile phase consisted of acetonitrile and 4% acetic acid （60：40, V/V） and the flow rate was set at 1.0 ml/min. Under the column temperature of 30 ℃, the typical injection volume was rated at 20μl. Results Curcumin showed prefer- ential linearity in the range of 0.050-40.0 μg/mL （r=0.9998, n=8）. The entrapment efficiency of three batches of sam- ples was 98.11%, 97.18% and 98.59%, with the drug-loading efficiency of 3.89%, 3.76% and 4.12%, respectively. Conclusion Our established methodology is featured by high specificity and convefiience and appears applicable for determination of drug contents and entrapment efficiency of curcumin nanostructured lipid carriers.