| 抗GSTAl单克隆抗体的制备与鉴定 |
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| 引用本文： | 王志刚,乌云达来,关静. 抗GSTAl单克隆抗体的制备与鉴定[J]. 内蒙古医学杂志, 2007, 39(9): 1038-1042 |
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| 作者姓名： | 王志刚 乌云达来 关静 |
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| 作者单位： | 赤峰市医院药局,内蒙古,赤峰,024000;赤峰市医院药局,内蒙古,赤峰,024000;赤峰市医院药局,内蒙古,赤峰,024000 |
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| 摘 要： | 目的:制备鼠抗人谷胱甘肽S转移酶A1(GSTAI)的单克隆抗体(mAb),并进行特性鉴定。方法:用纯化后的GSTAl融合蛋白作为抗原免疫BALB/c小鼠,采用常规的细胞融合技术制备抗GSTAl的单克隆抗体(mAb)。用ELISA法测定鼠抗人GSTA1血清的效价;用Western blot方法鉴定抗血清的特异性。结果:筛选到l株可稳定分泌抗人GSTAlmAb的细胞株。腹水效价为12 800。Western blot显示在相对分子量(Mr)约25KD处出现特异性条带。说明制备的mAb可特异地识别成人肝细胞中相对分子量(Mr)约25KD的GST-GSTA1。结论:获得一株能特异性识别抗人GSTAI的单克隆抗体,可用于肝损伤性疾病的诊断。
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| 关 键 词： | 谷胱甘肽S转移酶Al 单克隆抗体鉴定 Western blotting |
| 文章编号： | 1004-0951（2007）09-1038-05 |
| 修稿时间： | 2007-06-15 |
Preparation and Identification of the Monoclonal Antibody Against Humam |
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| AHANG Zhi-gang,WU YUN DA LAI,GUAN Jing. Preparation and Identification of the Monoclonal Antibody Against Humam[J]. Inner Mongolia Medical Journal, 2007, 39(9): 1038-1042 |
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| Authors: | AHANG Zhi-gang WU YUN DA LAI GUAN Jing |
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| Affiliation: | Department of Pharmacy, Chifen city Hospital, Chifen 024000 China |
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| Abstract: | Objective:To prepare the mouse monoclonal antibodies against human GSTAl and characterize its properties.Methods: Used GST-GSTA1 as the immunogen to prepare the mouse monoclonal antibodies.The properties of antiserum against human GSTAl were characterized by ELISA and Western blotting.Results: Acquired one clone of cell that can excrete monoclonal antibodies against human GSTAl stably.The GST-GSTA1 fusion protein was highly expressed with a molecular weight of 25 KDa,The titre of ascites against human GSTAl was l:12 800.Conclusions:The successful generation monoclonal antibodies the GST-GSTAl fusion protein will provide efficient affinity reagents for the further functional studies of GSTAl expressed in human hepatocellular impairment. |
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| Keywords: | Western blotting |
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