大鼠嗅鞘细胞体外培养及免疫组化研究

目的：建立体外纯化培养嗅鞘细胞（olfactory ensheathing cells，OECs）的稳定方法并观察培养嗅鞘细胞（OECs）形态学特征。方法：原代培养成年雄性SD大鼠嗅球的嗅神经层和颗粒层中提取的OECs，利用差速贴壁和阿糖胞苷（Ara-c）抑制法除去混杂的成纤维细胞和星形胶质细胞以获得纯化的OECs。用P75抗体免疫组织化学染色鉴定OECs，计算纯化度。结果：此方法获得的OECs纯度可达93％以上。OECs的外形主要以突起细长的双极和三极为主，或无突起呈“煎蛋样”，随时间延长细胞生长排列呈现一定方向性。结论：此方法稳定易复制，可获得高纯度的OECs。

Culture in vitro and immunohistochemical investigation of rat olfactory ensheathing cell

Objective: To establish steady method of culturing and purifying the olfactory ensheathing cells(OECs) in vitro and investigate the morphological character of cultured OECs. Methods: The OECs were detached from the outer olfactory nerve and glomerular layers of the olfactory bulb of the SD adult rat. The primary cultured cells were purified by different attachable speed and Cytosine Arabinoside inhibition to dissociate fibroblasts and astrocytes. The purity of the OECs was evaluated according to the percentage of P75-immunostaining cells. Results: Purity of the OECs exceeded 93% with this culture method. Bipolar and tripolar cells with long and slim enations, or nonpolar omelette-like cells were the main morphology of the OECs. Along culturing time prolongation, the OECs grew in a certain direction. Conclusion: Present method was effective for acquiring high purity OECs and easily replicated.