Influence of tissue nitration on tissue damage with thermal injury |
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Authors: | Hajime Inoue Miyuki Tomioka Masanobu Shimokawa Hiroyuki Nishikawa Ryoko Kojima Norio Kumagai |
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Institution: | (1) Institute of Advanced Medical Science, St. Marianna University Graduate School of Medicine, 2-16-1 Sugao, Miyamae Kawasaki, 216-0015, Japan;(2) Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae Kawasaki, 216-0015, Japan |
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Abstract: | A gas mediator, nitric oxide, is converted into peroxynitrite in the presence of superoxide anion. Peroxynitrite is a potent
oxidant, which injures various tissues and organs by nitration of tyrosine residue in protein and enhances the inflammatory
response in the prolonged phase. In this study, the authors investigated the relationship between peroxynitrite-mediated tissue
nitration and tissue damage with thermal injury using an experimental burn model. The content of nitrotyrosine in the burned
tissue significantly increased 1 to 6 h after injury. The nitrotyrosine content in the burned ear significantly decreased
with 100 mg/kg of LNAME administration. Vascular hyperpermeability was also significantly suppressed in the iNOS antibody
immunoneutralized mice 6 h after injury. There was a positive correlation between the severity of tissue damage, an indicator
of which is the increase in the weight of the burned ear along with the development of edema after injury, and the concentration
of nitrotyrosine in the wound tissues. Nitrotyrosine-like immune reactants were also diffusely detected in the burned region
and the surrounding areas. These results indicate that peroxynitrite is produced in the surrounding burned region and a reaction
of nitration of tissue tyrosine is involved with tissue damage at the burn wound. Therefore, to prevent the systemic vascular
hyperpermeability and tissue damage in a large area burn or severe burn patients, the administration of NOS inhibitors or
radical erasers may be easy to manage generally by inhibition of peroxynitrite formation. |
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Keywords: | Nitrotyrosine Thermal injury Tissue damage Peroxynitrite |
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