| 中成药中黄曲霉毒素B1含量测定方法的探讨 |
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| 引用本文： | 钱维清,潘维芳.中成药中黄曲霉毒素B1含量测定方法的探讨[J].中国药品标准,2000,1(4):59-61. |
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| 作者姓名： | 钱维清 潘维芳 |
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| 作者单位： | 上海市药品检验所，上海市药品检验所 上海 200233，上海 200233 |
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| 摘 要： | 目的：考察中成药中污染黄曲霉毒素B1(AFB1)的情况．为制定标准和检测方法提供依据。方法：应用酶联免疫吸附分析法(ELISA)、薄层色谱法(TLC)对27个品种共44批霉菌检验不合格的中成药进行AFB1测定。结果：ELISA法检出率为100％，TLC法检出率为36％。结论：证明两种方法检出率有较大差异。结论：(ELISA)法灵敏，准确，适合中成药中污染AFB1的定量分析用。
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| 关 键 词： | 中成药 黄曲霉毒素B1 含量 测定 |
METHODOLOGICAL EXPLORATION OF THE ASSAY FOR AFLATOXIN B1 IN CHINESE PATENT PREPARATIONS |
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| Authors: | Qian Weiqing and Pan Weifang |
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| Institution: | Shanghai Institute for Drug Control, Shanghai, 200233;Shanghai Institute for Drug Control, Shanghai, 200233 |
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| Abstract: | A Objective: To investigate the Chinese patent preparations contaminated by aflatoxin B_1 so that provide a scientific basis for technical specifications on drug quality. Method: Enzyme linked immunosorbent assay (ELISA) and thin layer chromatography (TLC)were applied to the assay for aflatoxin B_1in 44 batches of 27 Chinese patent preparations contaminated by mould. Result: Detectable rates of ELISA and TLC are 100% and 36% respectively, Conclution. (?) |
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| Keywords: | Aflatoxin B_1 Enzyme linked immuno-sorbent assay(ELISA) thin layer chromatography (TLC) |
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