| 多重耐药铜绿假单胞菌随机扩增多态性DNA的分型及应用 |
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| 引用本文： | 王苏建,王家平,王仕忠,鲁科峰,钱丽萍.多重耐药铜绿假单胞菌随机扩增多态性DNA的分型及应用[J].检验医学与临床,2005,2(3):97-98,101. |
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| 作者姓名： | 王苏建 王家平 王仕忠 鲁科峰 钱丽萍 |
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| 作者单位： | 南京医科大学附属常州第二人民医院检验科,213003,江苏;南京医科大学附属常州第二人民医院检验科,213003,江苏;南京医科大学附属常州第二人民医院检验科,213003,江苏;南京医科大学附属常州第二人民医院检验科,213003,江苏;南京医科大学附属常州第二人民医院检验科,213003,江苏 |
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| 摘 要： | 目的 应用随机扩增多态性DNA（RAPD）技术，对多重耐药铜绿假单胞菌（MRPA）进行基因多态性研究。方法对临床分离的32株MRPA进行随机引物PCR扩增，扩增产物进行电泳和聚类分析。结果 32株MRPA通过RAPD分型可分为19个基因型。耐药表型相同，基因型可相同；但绝大多数则不同。结论 通过RAPD分析，了解MAPA基因型特性，明确其耐药表型与基因型的关系，并为该菌的感染控制提供分子流行病学依据。
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| 关 键 词： | 多重耐药 多态现象(遗传学) 假单胞菌 铜绿 DNA |
| 收稿时间： | 2004-10-19 |
| 修稿时间： | 2004-10-19 |
Application and genotyping of multi-resistant pseudomonas aeruginosa by randomly amplified polymorphic DNA |
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| Wang SuJian;Wang GuPing;Wang ShiZhong;Lu KeFeng;Qian LiPing.Application and genotyping of multi-resistant pseudomonas aeruginosa by randomly amplified polymorphic DNA[J].Laboratory Medicine and Clinic,2005,2(3):97-98,101. |
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| Authors: | Wang SuJian;Wang GuPing;Wang ShiZhong;Lu KeFeng;Qian LiPing |
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| Abstract: | Objective To investigate the genetic Polymorphism of meticillin-resistant Pseudomonas aeruginosa(MRPA)by randomly amplified polymorphic DNA(RAPD).Methods The genes of MRPA were amplified by RAPD assay in 32 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis.Results 32 strains MRPA are classified into 19 genetypes,the phenotypes of drug resistance are the same,but most of the genetypes are different even though some of them can be equal.Conclusion RAPD assay reveals the special genetypes of MRPA and the relationship between the phenotype and genetype of drug resistance definite,which provides molecular epidemiological evidence for strategies to control MRPA infections. |
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| Keywords: | Meticillin-resistant Polymorphicsm genetics Pseudomonas aeruginosa aeruginosa DNA |
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