慢性髓系白血病患者各期id4基因甲基化状态研究

本研究探讨id4基因在慢性髓系白血病（CML）不同时期甲基化状态及其与疾病进展的关系。采用甲基化特异的PCR（MS-PCR）方法对48例CML患者和10例正常人的骨髓标本进行id4基因启动子区甲基化状态检测。结果表明：id4基因在正常人及CML慢性期患者骨髓中呈现完全非甲基化状态,而在CML急变期（包括加速期）骨髓中id4甲基化率为66%,较正常人和CML慢性期明显增加,二者差异具有统计学意义（p〈0.05）。系列研究结果也显示,同一CML患者的id4基因甲基化状态在慢性期时是非甲基化的,而在加速期或急变期却为甲基化状态。结论：id4基因在CML慢性期呈非甲基化,在加速期或急变期则呈甲基化状态,因而检测id4基因甲基化程度有助于监测CML患者的病情演变,可作为CML疾病进展的标志。

Methylation Status of id4 Gene Promoter in Patients with Chronic Myeloid Leukemia

This study was purposed to investigate the methylation status of id4 gene promoter in patients with chronic myeloid leukemia（CML） and explore the relationship between methylation of the id4 gene and progress of CML. The methylation status of id4 gene in 48 chronic myeloid leukemia patients and 10 healthy individuals was detected by using methylation-specific polymerase chain reaction （MS-PCR）. The results showed that id4 gene was unmethylated in bone marrow samples from both healthy individuals and CML patients in chronic phase （CP）. The rate of id4 gene methylation in both CML patients in accelerated phase （AP） and blast crisis （BC） was 66%, and was higher than those of CML patients in CP phase. There was significant difference between them （p0.05）. In one CML patient who received a serial observations, the status of id4 was unmethylated in CP, but it was methylated in AP and BC phase. It is concluded that the id4 gene in CML patients is unmethylated in CP, while it is methylated in AP or BC. The detection of id4 gene methylation status may be useful for monitoring disease advance in CML and may be used as a marker of disease progression in CML.