| 螺旋霉素与乙酰螺旋霉素的薄层色谱定量分析 |
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| 引用本文： | 孙成,于如嘏,杨清华,盛曙光,赵霞芬.螺旋霉素与乙酰螺旋霉素的薄层色谱定量分析[J].药学学报,1987,22(7):515-519. |
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| 作者姓名： | 孙成 于如嘏 杨清华 盛曙光 赵霞芬 |
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| 作者单位： | 中国药科大学，中国药科大学，中国药科大学，江苏省药品检验所，江苏省药品检验所 南京 1985届研究生，南京，南京，南京，南京 |
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| 摘 要： | 用薄层色谱法考察了NaOH浓度对分离的影响,选用了含0.3 mol/L NaOH的0.5%CMC-Na溶液调和制板,以甲醇—乙酸乙酯—环己烷(10:45:45)为展开剂可将螺旋霉素完全分离,以甲醇—乙酸乙酯—环己烷(8:46:46)为展开剂可将乙酰螺旋霉素基本分离。用CS-910薄层扫描仪对样品进行了定量分析。
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| 关 键 词： | 薄层色谱 乙酰螺旋霉素 二乙酰螺旋霉素 螺旋霉素 |
| 收稿时间： | 1986-05-07 |
QUANTITATIVE ANALYSES OF SPIRAMYCIN AND ACETYLSPIRAMYCIN BY THIN-LAYER CHROMATOGRAPHY |
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| SUN Cheng,YU Ru-Gu,YANG Qing-Hua,SHENG Shu-Guang and ZHAO Xia-Fen.QUANTITATIVE ANALYSES OF SPIRAMYCIN AND ACETYLSPIRAMYCIN BY THIN-LAYER CHROMATOGRAPHY[J].Acta Pharmaceutica Sinica,1987,22(7):515-519. |
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| Authors: | SUN Cheng YU Ru-Gu YANG Qing-Hua SHENG Shu-Guang and ZHAO Xia-Fen |
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| Abstract: | Thin-layer chromatography was used for separation of spiramycin (SPM) and acetylspiramycin (Ac-SPM). 0.3 mol/L NaOH in 0.5% CMC-Na solution was selected to spread plates with silica gel (3.3:1), methanol—ethyl acetatecyclohexane (10:45:45) and (8:46:46) were employed for separating SPM and AcSPM respectively. It has been found that the content of each component in SPM made in China is different from that made in Japan, namely, in the former SPM Ⅱ, Ⅲ are the chief components, but in the latter SPM, SPM Ⅰ is the chief one. Moreover, in Ac-SPM made in China, diacetylspiramycin Ⅱ, Ⅲ are dominant and in Ac-SPM made in Japan, di- and monoacetylspiramycin Ⅱ are the principal components. |
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| Keywords: | Thin-layer Chromatography Acetylspiramycin Diacetylspiramycin Spiramycin |
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