诱导小鼠肝硬化对结肠癌肝转移的抵抗作用

目的:研究诱导小鼠肝硬化对结肠癌肝转移的抵抗作用及其可能的机制。方法:诱导小鼠肝硬化及结肠癌肝转移模型,比较正常肝与肝硬化小鼠肝转移率;免疫组化法测定层粘连蛋白(laminin,LN)、纤维连接蛋白(fibronectin,FN)及E-选择素(E-selectin)在小鼠肝脏的表达。结果:①肝硬化小鼠肝转移率(4/16,25%)低于正常肝小鼠(14/16,87.5%)(P=0.001);②肝硬化后移植瘤小鼠LN、FN表达高于正常肝移植瘤小鼠,E-selectin表达低于正常肝移植瘤小鼠。结论:动物实验证实肝硬化对结肠癌肝转移有抵抗作用;LN、FN及E-selectin在此过程中可能起一定作用。

Induced cirrhosis liver is resisitant to liver metastasis from colorectal cancer in mice

Purpose:To study the resistance of induced mice cirrhosis to liver metastasis from colorectal cancer and its possible mechanism s. Methods:Developed a mouse model of liver cirrhosis and liver m etastases from colorectal cancer,observed the incidence of liver metastasis in cirrhosis liver mice and normal liver mice;examined the expression of liminin( LN),fibronectin(FN) and E-selectin in livers by immunohistochemistry(IH). Results:The incidence of liver metastasis in cirrhotic mice (4 of 16, 25%) was significantly lower(P= 0.001) than that in normal liver mice (14 of 16, 87.5%). The expression of liminin,fibronectin in cirrhosis mice were significantly higher than that in normal liver mice inoculated with tumor, the e xpression of E-selectin in cirrhotic mice was significantly lower than that in normal liver mice inoculated with tumor. Conclusions:It was comfirmed that cirrhotic liver is resistant to the development of liver metastasis from colorectal cancer through animal exp eriments.There were eridence that liminin,fibronectin and E-selectin played imp ortant roles in the process.