人参低聚肽对急性酒精性肝损伤大鼠的保护作用

目的 探讨人参低聚肽（GOP）对酒精诱导的大鼠急性肝损伤的保护作用及其可能机制。方法 采用SPF级SD雄性大鼠,随机分为7组,每组10只,包括，1个空白对照组、1个模型对照组、1个乳清蛋白对照组（0.250 0 g/kg）和4个GOP剂量组(0.062 5、0.125 0、0.250 0、0.500 0 g/kg)。连续灌胃30d后,采用体积分数 50%的酒精以 7g/(kg·BW)剂量灌胃造成大鼠急性酒精性肝损伤模型,测定大鼠血清ALT、AST活性和肝脏SOD、GSH、GSH-PX及MDA含量。结果 与模型对照组相比,GOP干预可以显著降低大鼠血清ALT、AST和肝组织MDA(P<0.05或P<0.01)的水平,增加肝组织SOD 、GSH和GSH-PX的活性（P<0.05或P<0.01)。结论 GOP对酒精诱导引起的大鼠急性肝损伤具有保护作用,其机制可能与提高机体抗氧化水平有关。

Protective effects of panax ginseng oligopeptide on acute alcohol-induced liver injury and underlying mechanism

Objective To investigate the effects and mechanism of panax ginseng oligopeptide(GOP)on acute alcohol-induced Liver injury in rats. Methods A total of 70 SD rats were randomly assigned to 7 groups,including a normal control group,a model group,a whey protein group(0.2500 g/kg),and 4 GOP intervention groups (0.0625,0.1250,0.2500,0.5000 g/kg).7 g/kg/BW 50% v ethanol was given intragastricly after the 30 days of rat stomach-lavaging continuously,serum markers were assayed,and the oxidative stress and lipid peroxidation parameters in liver tissue were conducted. Results We found that the GOP treatment prevented the elevation of serum aminotransferase,and SOD,GSH,GSH-PX contents in all test groups were increased significantly (P<0.05 or P<0.01).In addition,MDA contents decreased significantly in all GOP prevention groups (P<0.01). Conclusion These findings suggest that GOP have a significant protective effect on acute alcohol-induced Liver injury in rats,and the underlying mechanism may be related to enhancing anti-oxidative activities.