蛋白激酶SGK3与乳腺癌临床病理相关性研究

摘要:目的 通过对不同类型乳腺组织和乳腺癌中SGK3的表达情况进行分析，初步探讨SGK3与乳腺癌临床病理特征的相关性。方法 利用免疫组化方法，检测SGK3在正常乳腺组织和乳腺癌组织中的表达，进一步研究SGK3在不同类型乳腺癌以及同一类型不同病理分级乳腺癌组织中的表达情况，并结合临床参数探讨SGK3与乳腺癌临床病理特征的关系。 结果 （1）免疫组化结果显示，乳腺癌组织中蛋白激酶SGK3表达量增高，与正常乳腺组织和癌旁组织相比，有差异（P值分别为0.007,0.016）。（2）SGK3在乳腺纤维腺瘤、叶状囊肉瘤以及导管内癌的表达量极低，与正常乳腺组织相比差异无统计学意义（P值分别为0.076,0.365,0.500）；而在浸润性导管癌中的表达明显增高（P值为0.041）。（3）SGK3的表达不受浸润性导管癌的分期影响，但与ER受体表达密切相关，雌激素受体（ER）阳性的浸润性导管癌与ER阴性的浸润性导管癌相比组织中SGK3的表达量明显增高（P值为0.033）。结论 SGK3在乳腺浸润性导管癌组织中呈高表达，且在ER阳性的润性导管癌组织中的表达高于ER阴性组织，SGK3有可能成为乳腺浸润性导管癌临床诊断和预后的指标。

Correlation Study between Protein Kinase SGK3 and Clinical Pathological in Breast Cancer

Abstract:Objective This study was to investigate the correlation between serum glucocorticoid-regulated kinase 3 (SGK3) and clinical pathological features of breast cancer. Methods Tissue microarray technique was used to detect the expression of SGK3 in normal breast tissue and breast cancer tissue. The expression of SGK3 in different breast cancer tissue and different pathological grade breast cancer tissue was studied by immunohistochemical method. Results The expression of SGK3 significantly increased in breast cancer tissue in comparison to the normal breast tissue and the adjacent tissue (P=0.007 and 0.016, respectively). The expression of SGK3 was very low in breast fibroadenoma, cystosarcoma phyllodes and intraductal carcinoma, without significant difference from the normal breast tissue (P=0.076, 0.365, 0.500, respectively). However, the expression of SGK3 in invasive ductal carcinoma increased significantly in comparison to the normal breast tissues and adjacent tissues (P=0.041). The expression of SGK3 was not affected by the stage of invasive ductal carcinoma, but it was closely related with the expression of ER receptor. The expression of SGK3 in the tissue of the estrogen receptor positive (ER+) invasive ductal carcinoma was significantly increased in comparison to that of the estrogen receptor negative (ER-) one (P=0.033). Conclusion SGK3 was highly expressed in breast invasive ductal carcinoma tissues, and positively correlated with the pathological grade of tumor and the estrogen receptor expression. Therefore, SGK3 may be a potential target for clinical diagnosis and prognosis of breast invasive ductal carcinoma.