肥胖小鼠血清BMP4/7与炎症因子分布特征和指标间的相关性

目的 观察肥胖小鼠体内动脉血管与脂肪组织中骨形态发生蛋白-4、7(BMP4、BMP7)的表达,研究血清BMP4/BMP7比率变化与动脉血管炎症相关性。方法 以ob/ob小鼠为肥胖组,C57BL/6小鼠为正常组,每组各8只。普通饮食喂养12周后,心脏取血并处死,分别取腹主动脉与腹部脂肪组织,用荧光定量PCR和Western blot检测各组织中BMP4、BMP7、IL-1β、IL-9及p-Smad1 mRNA与蛋白的表达。结果 肥胖组血清中BMP4的表达水平显著高于正常组(P<0.05),BMP7的表达两组无明显差异,肥胖组血清BMP4/BMP7比率显著高于正常组(P<0.05);肥胖组BMP4 mRNA与蛋白在动脉血管的表达高于正常组,在脂肪组织中的表达少于正常组(P<0.05)。BMP7 mRNA与蛋白在动脉血管与脂肪组织中的表达均无显著差异。肥胖组在动脉血管处BMP4mRNA/BMP7mRNA比率显著高于正常组(P<0.05),而在脂肪组织中的表达二者差异不明显。IL-1β和IL-9 mRNA与二者蛋白在动脉血管与脂肪组织中的表达水平均高于正常组(P<0.05)。结论 肥胖小鼠体内动脉血管的炎症反应明显,与血清BMP4/BMP7比率增加相关。

Correlation between serum BMP4/BMP7 and inflammatory factor distribution and indicators in obese rats

AIM To investigate the expression of BMP4 and BMP7 in arteries and fat tissue and the relationship between arterial vascular inflammation and ratio changes of serum BMP4 / BMP7 in obese mice. METHODS Eight 4-week-old ob / ob mice were used as the obese group and eight C57 BL /6 mice were used as the normal group. After a 12-week full diet,the blood,abdominal aorta and inguinal adipose tissue were collected after all animals were sacrificed and the expressions of protein and mRNA of BMP4,BMP7,IL-1β,IL-9 and p-Smad1 were detected using quantitative PCR and Western blotting. RESULTS Serum expression of BMP4 in the obese group was significantly higher than in the normal group and no significant changes in BMP7 expression were found between groups. Ratio of BMP4 / BMP7 in the obese group was significantly higher than in the normal group( P < 0. 05). Expressions of BMP4 protein and mRNA level in the arteries in obese group were higher than those in the normal group but were lower in adipose tissues in obese group. BMP7 protein and mRNA expressions showed no clear difference in arteries and adipose tissues between groups. Ratio of BMP4 mRNA / BMP7 mRNA showed obvious difference in the arteries between groups( P < 0. 05),but no significant difference in adipose tissue was observed between groups. Inflammations measured by mRNA and protein expressions of IL-1β and IL-9 in the arteries and obesity in obese group were higher than in the normal group( P < 0. 05). CONCLUSION There is obvious inflammation in the artery of obese mice,which may be related to the increasing ratio of BMP4 / BMP7.