湖北海棠叶总黄酮对日本血吸虫感染小鼠肝纤维化的抑制作用

目的探讨湖北海棠叶总黄酮对日本血吸虫感染所致小鼠肝纤维化的保护作用。方法随机将未感染和感染日本血吸虫尾蚴昆明小鼠分成A～F6组。即未感染空白对照组（A）、模型组（B）、复方鳖甲软肝片阳性组（C）、湖北海棠叶总黄酮治疗高剂量114mg（/kgd）]组（D）、中剂量57mg（/kgd）]组（E）、低剂量28.5mg（/kgd）]组（F）,每组10只。感染后第42天,C、D、E、F组均用吡喹酮驱虫治疗,500mg/（kgd）2日疗法,之后各组开始灌胃给药持续治疗60d,A组和B组生理盐水灌胃对照。实验结束处死小鼠,留取肝脏组织和血清,测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、透明质酸（HA）含量,计算肝体指数,测定肝组织中羟脯氨酸（HYP）。苏木素伊红和Masson胶原纤维染色观察小鼠肝组织变性、虫卵肉芽肿与胶原沉积等病理改变。结果与A组相比,B、C、D、E、F组小鼠肝脏有明显虫卵肉芽肿形成、胶原沉积与肝纤维化,伴有不同程度肝细胞炎性损伤坏死。血清中ALT、AST、HA和肝组织中HYP含量均明显升高（P〈0.05或P〈0.01）。与B组相比,湖北海棠叶总黄酮高、中、低剂量组ALT、AST、HA和HYP含量及肝体指数均明显降低（P〈0.05或P〈0.01）,肝组织坏死与胶原沉积明显减轻,虫卵肉芽肿面积减小。结论湖北海棠叶总黄酮具有抗血吸虫性肝纤维化作用。

Inhibitory effect of total flavonoids of Malus hupehensis on hepatic fibrosis induced by Schistosoma japonicum in mice

Objective To investigate the effects of total flavonoids of Malus hupehensis on hepatic fibrosis in mice induced by Schistosoma japonicum.Methods The mice model of hepatic fibrosis which infected by cercariae of S.japonicum were randomly divided into 6 groups：Group A as a blank control,Group B as a model,Group C as a positive control by complex liver soften tablet of turtle,Group D,E,F treated with a high dose of 114 mg/（kg-]d）,middle dose of 57 mg/（kg-]d）,and low dose of 28.5 mg/（kg-]d） of total flavonoids of Malus hupehensis,respectively.Every group had 10 mice.Each group of C,D,E,F was orally given praziquantel at a dose of 500 mg/（kg-]d） for 2 d,on 42 d after the infection,and then administered with total flavonoids of Malus hupehensis for 60 d.Group A and B were orally given with sodium chloride.All the mice were killed at the end of the administration.Serum hyaluronic acid（HA）,alanine aminotransferase（ALT） and aspartate aminotransferase（AST） were detected;hydroxyproline in liver tissues was detected;areas of egg granuloma and degrees of hepatic fibrosis were observed via HE and Masson staining.Results Compared with the blank control Group A,the egg granuloma appeared obviously,the collagen deposit and fibrosis occurred in liver tissues of Group B,C,D,E,F.The levels of ALT,AST,HA in sera and HYP in liver tissues were significantly higher（P0.05 or P0.01）.However,the levels of ALT,AST,HA and HYP in the high,middle and low dose groups of total flavonoids were significantly lower than those in the model Group B（P0.05 or P0.01）,the areas of egg granuloma,the collagen deposits and the degrees of hepatic fibrosis in Group C,D,E,F were significantly lower than those in the model Group B.Conclusion The total flavonoids of Malus hupehensis have an obviously inhibitory effect on the hepatic fibrosis induced by Schistosoma japonicum infection.