新型抗钙化牛颈静脉带瓣管道的实验研究

目的探索对戊二醛（glutaraldehyde,GA）固定的牛颈静脉改性的新抗钙化处理方法,以期研制出一种新型抗钙化牛颈静脉带瓣管道（BJVC）。方法取新鲜牛颈静脉,依次经戊二醛、Triton X-100和环氧氯丙烷（epoxychloropropane,EC）处理,制备出新型抗钙化牛颈静脉带瓣管道。分别比较新鲜组、单纯戊二醛处理组（GA组）和新型抗钙化组（EC＋Tr组）牛颈静脉管道的大体形态、组织学特点、热皱缩温度、拉伸强度和断裂伸长率。建立大鼠皮下包埋动物模型,以GA组为对照,通过大体观察、HE染色和原子吸收光谱仪测定钙含量,评价EC＋Tr组血管片包埋8周后的抗炎、抗钙化性能。结果 EC＋Tr组牛颈静脉管道的热皱缩温度、拉伸强度和断裂伸长率均较新鲜组显著提高（86.15±0.92℃vs.69.94±0.92℃,t=35.239,P=0.000;5.31±0.14mPa vs.3.15±0.95mPa,t=6.362,P=0.000;265.11%±27.80%vs.165.21%±25.06%,t=7.550,P=0.000）;以上指标EC＋Tr组亦明显高于GA组（86.15±0.92℃vs.82.73±1.28℃,t=6.137,P=0.000;5.31±0.14mPa vs.4.52±0.56mPa,t=3.871,P=0.002;265.11%±27.80%vs.237.85%±17.41%,t=2.351,P=0.034）。而动物皮下包埋结果显示：EC＋Tr组血管片结构致密,炎性细胞浸润少;EC＋Tr组的钙含量明显低于GA组（51.22±2.69μg/mg vs.73.24±3.82μg/mg,t=11.545,P=0.000）。结论通过Triton X-100、EC对戊二醛固定的牛颈静脉进行联合改性处理,可显著提高牛颈静脉管道的生物力学性能,并延缓其体内钙化进程,是一种有效的、抗钙化的牛颈静脉带瓣管道处理方法

A New Type of Anticalcification Bovine Jugular Venous Conduit

Objective To evaluate a new type of treatment that reduces calcification of glutaraldehyde-treated bovine jugular venous conduit(BJVC).Methods Fresh bovine jugular veins were treated with glutaraldehyde,followed by Triton X-100 and epoxy chloropropane(EC+Tr group).We compared the group's appearance,histology,shrinkage temperature,tensile strength,and elongation at break with those of a fresh group,and with a group treated with glutaraldehyde only(GA group).We then implanted the EC+Tr and GA group BJVCs subc...