| 纤维素酶提高胡芦巴有效成分收率的研究 |
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| 引用本文： | 姜彬慧,赵余庆,郑丹,荆宇,胡筱敏,郑龙熙.纤维素酶提高胡芦巴有效成分收率的研究[J].中草药,2004,35(6):626-629. |
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| 作者姓名： | 姜彬慧 赵余庆 郑丹 荆宇 胡筱敏 郑龙熙 |
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| 作者单位： | 1. 东北大学资源与土木工程学院,辽宁,沈阳,110004
2. 辽宁中医学院,辽宁,沈阳,110032 |
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| 摘 要： | 目的利用纤维素酶提高胡芦巴有效成分的收率。方法以甾体皂苷收率为指标，确定提高胡芦巴有效成分收率的最佳酶及最佳酶解条件。结果实验组有效成分的收率约是对照组的3．6倍；酶解的最佳实验条件为：温度50℃，pH4．4，反应72h。结论利用纤维素酶可以提高胡芦巴有效成分——甾体皂苷的收率。
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| 关 键 词： | 胡芦巴 纤维素酶 甾体皂苷 |
| 收稿时间： | 2003/12/11 0:00:00 |
Studies on raising yield of effective constituent in Trigonella foenum-graecum by cellulase |
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| JIANG Bin-hui,ZHAO Yu-qing,ZHENG Dan,JING Yu,HU Xiao-min and ZHENG Long-xi.Studies on raising yield of effective constituent in Trigonella foenum-graecum by cellulase[J].Chinese Traditional and Herbal Drugs,2004,35(6):626-629. |
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| Authors: | JIANG Bin-hui ZHAO Yu-qing ZHENG Dan JING Yu HU Xiao-min and ZHENG Long-xi |
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| Institution: | College of Resources and Civil Engineering, Northeast University, Shenyang 110004, China;Liaoning College of Traditional Chinese Medicine, Shenyang 110032, China;College of Resources and Civil Engineering, Northeast University, Shenyang 110004, China;Liaoning College of Traditional Chinese Medicine, Shenyang 110032, China;College of Resources and Civil Engineering, Northeast University, Shenyang 110004, China;College of Resources and Civil Engineering, Northeast University, Shenyang 110004, China |
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| Abstract: | Object To improve the yield of the effective constituent in Trigonella foenum-graecum L.by cellulase. Methods To take the yield of saponin as index to confirm the optimal enzyme and the best enzymolysis condition for improving the yield of saponin. Results The yield of the effective constituent in experimental group is about 3.6 times of that in control group; the best experimental condition for enzymolysis is: temperature 50 ℃, pH 4.4, time 72 h. Conclusion By using cellulase, the yield of effective constituent of T. foenum-graecum - saponin could be increased. |
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| Keywords: | Trigonella foenum-graecum L cellulase saponin |
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